論文

査読有り
2004年10月

Delivery of cytolethal distending toxin B induces cell cycle arrest and apoptosis in gingival squamous cell carcinoma in vitro

EUROPEAN JOURNAL OF ORAL SCIENCES
  • K Yamamoto
  • ,
  • K Tominaga
  • ,
  • M Sukedai
  • ,
  • T Okinaga
  • ,
  • K Iwanaga
  • ,
  • T Nishihara
  • ,
  • J Fukuda

112
5
開始ページ
445
終了ページ
451
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/j.1600-0722.2004.00157.x
出版者・発行元
BLACKWELL MUNKSGAARD

The cytolethal distending toxin (Cdt) from Actinobacillus actinomycetemcomitans consists of three proteins, CdtA, CdtB, and CdtC, which are responsible for cell cycle arrest and apoptosis. In the present study, local delivery systems of recombinant CdtB and CdtB-expressing plasmid were established using Ca9-22, human gingival squamous cell carcinoma cell line. When CdtB was delivered to Ca9-22 cells using a BioPORTER, a 32-kDa protein was detected by Western blotting, and G2 cell cycle arrest and apoptosis occurred. In addition, the CdtB delivered upregulated the expression of phosphorylated p53 and the cyclin-dependent kinase inhibitor p21(CIP1/WAF1) in Ca9-22 cells, suggesting that these intracellular molecules might contribute to the induction of G2 cell cycle arrest and apoptosis. When the CdtB-expressing plasmid was transfected into Ca9-22 cells by lipofection or electroporation, CdtB (32 kDa) was clearly detected. Further, TdT-mediated dUTP nick end labeling positive cells were observed after transfection of the CdtB-expressing plasmid. These findings indicated that delivery of the CdtB protein and transfection of the cdtB gene induced cell cycle arrest and apoptosis in Ca9-22 cells in vitro, and we conclude that it may be possible to induce apoptosis in human gingival squamous cell carcinoma by electroporation of the cdtB gene.

Web of Science ® 被引用回数 : 33

リンク情報
DOI
https://doi.org/10.1111/j.1600-0722.2004.00157.x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/15458505
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000224179900010&DestApp=WOS_CPL
ID情報
  • DOI : 10.1111/j.1600-0722.2004.00157.x
  • ISSN : 0909-8836
  • PubMed ID : 15458505
  • Web of Science ID : WOS:000224179900010

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