論文

査読有り
2013年12月

High-resolution crystal structure of copper amine oxidase from Arthrobacter globiformis: assignment of bound diatomic molecules as O-2

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
  • Takeshi Murakawa
  • ,
  • Hideyuki Hayashi
  • ,
  • Tomoko Sunami
  • ,
  • Kazuo Kurihara
  • ,
  • Taro Tamada
  • ,
  • Ryota Kuroki
  • ,
  • Mamoru Suzuki
  • ,
  • Katsuyuki Tanizawa
  • ,
  • Toshihide Okajima

69
Pt 12
開始ページ
2483
終了ページ
2494
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1107/S0907444913023196
出版者・発行元
WILEY-BLACKWELL

The crystal structure of a copper amine oxidase from Arthrobacter globiformis was determined at 1.08 angstrom resolution with the use of low-molecular-weight polyethylene glycol (LMW PEG; average molecular weight similar to 200) as a cryoprotectant. The final crystallographic R factor and R-free were 13.0 and 15.0%, respectively. Several molecules of LMW PEG were found to occupy cavities in the protein interior, including the active site, which resulted in a marked reduction in the overall B factor and consequently led to a subatomic resolution structure for a relatively large protein with a monomer molecular weight of similar to 70 000. About 40% of the presumed H atoms were observed as clear electron densities in the F-o - F-c difference map. Multiple minor conformers were also identified for many residues. Anisotropic displacement fluctuations were evaluated in the active site, which contains a post-translationally derived quinone cofactor and a Cu atom. Furthermore, diatomic molecules, most likely to be molecular oxygen, are bound to the protein, one of which is located in a region that had previously been proposed as an entry route for the dioxygen substrate from the central cavity of the dimer interface to the active site.

Web of Science ® 被引用回数 : 9

リンク情報
DOI
https://doi.org/10.1107/S0907444913023196
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24311589
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000328370400021&DestApp=WOS_CPL

エクスポート
BibTeX RIS