論文

査読有り
2013年7月

Direct interactions between Z-DNA and alkaline earth cations, discovered in the presence of high concentrations of MgCl2 and CaCl2

JOURNAL OF INORGANIC BIOCHEMISTRY
  • Toshiyuki Chatake
  • ,
  • Tomoko Sunami

124
開始ページ
15
終了ページ
25
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jinorgbio.2013.03.004
出版者・発行元
ELSEVIER SCIENCE INC

In this study, crystals of Z-DNA hexamer d(CGCGCG) complexed with MgCl2 and CaCl2 were obtained in the presence of high concentrations of alkaline earth salts (500 mM) using a temperature control technique, and their crystal structures were determined at 1.3 angstrom resolution. Mg2+ and Ca2+ cations in these structures tend to interact directly with phosphate groups of Z-DNA duplexes: however, they tend to form water-mediated interactions with Z-DNA in the presence of lower concentrations of alkaline earth salts. In these crystals, a DNA duplex was laid along its c-axis and interacted with its 6 neighboring DNA duplexes through coordination bonds of P-O-center dot center dot center dot(Mg2+ or Ca2+)(center dot center dot center dot)O-P. A symmetrical hexagonal Z-DNA duplex assembly model may explain DNA condensation caused by alkaline earth salts. These structures offer insights into the functions of alkaline earth cations essential to the structures and assembly of Z-DNA duplexes. (C) 2013 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 10

リンク情報
DOI
https://doi.org/10.1016/j.jinorgbio.2013.03.004
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23578858
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000320087100003&DestApp=WOS_CPL

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