1987年
体外受精に由来する透明帯除去マウス胚の培養および移植成績
哺乳動物卵子学会誌
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- 巻
- 4
- 号
- 2
- 開始ページ
- 84
- 終了ページ
- 91
- 出版者・発行元
- 日本哺乳動物卵子学会
The aim of the present study was to determine the potential for the further developmentof zona-free mouse zygotes fertilized in vitro. Zona-pellucida of (C57BL/6J X C3H/HeN) F1 mouse eggs-were removed with 0.5% pronase 6 hr after insemination. Each one of zonafreeeggs were cultured for 114 hr in 20μ1 medium in Multiplate-Terasaki and 20μl and 100pl drops in dish. At 96 hr after insemination 58%, 74% and 66% of these eggs, respectively, developed into the blastocyst stage. After transfer to the uterus of foster-mothers, 4 (5%) of 76 blastocysts cultured in 20μl medium in Multiplate-Terasaki, developed into newborns.It was concluded that the zona-pellucida is not essential for development into the youngfrom the zygotes fertilized in vitro.
- リンク情報
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- CiNii Articles
- http://ci.nii.ac.jp/naid/130003839313
- ID情報
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- ISSN : 0289-5439
- CiNii Articles ID : 130003839313
- identifiers.cinii_nr_id : 9000254402662