論文

査読有り 国際誌
2018年12月30日

Single-step detection of norovirus tuning localized surface plasmon resonance-induced optical signal between gold nanoparticles and quantum dots.

Biosensors & bioelectronics
  • Fahmida Nasrin
  • ,
  • Ankan Dutta Chowdhury
  • ,
  • Kenshin Takemura
  • ,
  • Jaewook Lee
  • ,
  • Oluwasesan Adegoke
  • ,
  • Vipin Kumar Deo
  • ,
  • Fuyuki Abe
  • ,
  • Tetsuro Suzuki
  • ,
  • Enoch Y Park

122
開始ページ
16
終了ページ
24
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bios.2018.09.024

A new method of label free sensing approach with superior selectivity and sensitivity towards virlabel-freeon is presented here, employing the localized surface plasmon resonance (LSPR) behavior of gold nanoparticles (AuNPs) and fluorescent CdSeTeS quantum dots (QDs). Inorganic quaternary alloyed CdSeTeS QDs were capped with L-cysteine via a ligand exchange reaction. Alternatively, citrate stabilized AuNPs were functionalized with 11-mercaptoundecanoic acid to generate carboxylic group on the gold surface. The carboxylic group on the AuNPs was subjected to bind covalently with the amine group of L-cysteine capped CdSeTeS QDs to form CdSeTeS QDs/AuNPs nanocomposites. The fluorescence of CdSeTeS QDs/AuNPs nanocomposite shows quenched spectrum of CdSeTeS QDs at 640 nm due to the close interaction with AuNPs. However, after successive addition of norovirus-like particles (NoV-LPs), steric hindrance-induced LSPR signal from the adjacent AuNPs triggered the fluorescence enhancement of QDs in proportion to the concentration of the target NoV-LPs. A linear range of 10-14 to 10-9 g mL-1 NoV-LPs with a detection limit of 12.1 × 10-15 g mL-1 was obtained. This method was further applied on clinically isolated norovirus detection, in the range of 102-105 copies mL-1 with a detection limit of 95.0 copies mL-1, which is 100-fold higher than commercial ELISA kit. The superiority of the proposed sensor over other conventional sensors is found in its ultrasensitive detectability at low virus concentration even in clinically isolated samples. This proposed detection method can pave an avenue for the development of high performance and robust sensing probes for detection of virus in biomedical applications.

リンク情報
DOI
https://doi.org/10.1016/j.bios.2018.09.024
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30236804

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