論文

査読有り 国際誌
2019年9月11日

Characterization of the self-assembly of New Jersey polyomavirus VP1 into virus-like particles and the virus seroprevalence in Japan.

Scientific reports
  • Xianfeng Zhou
  • ,
  • Huimin Bai
  • ,
  • Michiyo Kataoka
  • ,
  • Masahiko Ito
  • ,
  • Masamichi Muramatsu
  • ,
  • Tetsuro Suzuki
  • ,
  • Tian-Cheng Li

9
1
開始ページ
13085
終了ページ
13085
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-019-49541-y

New Jersey polyomavirus (NJPyV) was discovered in 2014 in a pancreatic transplant recipient's vascular endothelial cells. Here, in the recombinant baculovirus system, VP1 protein of NJPyV expressed in insect cells was processed. The protein self-assembled into virus-like particles (NJPyV-LPs) in a cell-type-dependent manner, and the particles were then released into the culture media. Spherical ~50-nm-dia. NJPyV-LPs of uniform size with morphology resembling that of the native particles of polyomaviruses were purified from the fraction at 1.33 g/cm3 in supernatants of VP1-expressing Sf9 cells. We investigated the antigenic properties of purified NJPyV-LPs and performed a VLP-based enzyme immunoassay to determine the age-specific prevalence of NJPyV infection in a general Japanese population aged 1-70 years. The overall seropositivity rate of anti-NJPyV antibodies was only 1.8%. This might be explained by the low circulation of NJPyV in Japan. This is the first report of a large-scale serological survey of NJPyV in Asia (n = 1,050).

リンク情報
DOI
https://doi.org/10.1038/s41598-019-49541-y
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31511622
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739320

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