論文

査読有り 国際誌
2020年6月1日

Dual modality sensor using liposome-based signal amplification technique for ultrasensitive norovirus detection.

Biosensors & bioelectronics
  • Akhilesh Babu Ganganboina
  • ,
  • Ankan Dutta Chowdhury
  • ,
  • Indra Memdi Khoris
  • ,
  • Fahmida Nasrin
  • ,
  • Kenshin Takemura
  • ,
  • Toshimi Hara
  • ,
  • Fuyuki Abe
  • ,
  • Tetsuro Suzuki
  • ,
  • Enoch Y Park

157
開始ページ
112169
終了ページ
112169
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bios.2020.112169

Sensitive and accurate detection methods for infectious viruses are the pressing need for effective disease diagnosis and treatment. Herein, based on V2O5 nanoparticles-encapsulated liposomes (VONP-LPs) we demonstrate a dual-modality sensing platform for ultrasensitive detection of the virus. The sensing performance relies on intrinsic peroxidase and electrochemical redox property of V2O5 nanoparticles (V2O5 NPs). The target-specific antibody-conjugated VONP-LPs and magnetic nanoparticles (MNPs) enrich the virus by magnetic separation and the separated VONP-LPs bound viruses are hydrolyzed to release the encapsulated V2O5 NPs. These released nanoparticles from captured liposomes act as peroxidase mimics and electrochemical redox indicator resulting in noticeable colorimetric and robust electrochemical dual-signal. Utilizing the superiority of dual-modality sensor with two quantitative analysis forms, norovirus like particles (NoV-LPs) can be detected by electrochemical signals with a wide linear range and low detection limit. To verify the applicability in real samples, norovirus (NoV) collected from actual clinical samples are effectively-identified with excellent accuracy. This proposed detection method can be a promising next-generation bioassay platform for early-stage diagnosis of virus disease and surveillance for public health.

リンク情報
DOI
https://doi.org/10.1016/j.bios.2020.112169
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32250939

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