論文

査読有り 国際誌
2020年7月

Partial silencing of fucosyltransferase 8 gene expression inhibits proliferation of Ishikawa cells, a cell line of endometrial cancer.

Biochemistry and biophysics reports
  • Hana Shimoyama
  • ,
  • Toshiaki K Shibata
  • ,
  • Masahiko Ito
  • ,
  • Tomoaki Oda
  • ,
  • Toshiya Itoh
  • ,
  • Mari Mukai
  • ,
  • Madoka Matsuya-Ogawa
  • ,
  • Masashi Adachi
  • ,
  • Hirotake Murakami
  • ,
  • Takeshi Nakayama
  • ,
  • Kazuhiro Sugihara
  • ,
  • Hiroaki Itoh
  • ,
  • Tetsuro Suzuki
  • ,
  • Naohiro Kanayama

22
開始ページ
100740
終了ページ
100740
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrep.2020.100740

Endometrial cancer is the most common gynecologic malignancy and is associated with increased morbidity each year, including young people. However, its mechanisms of proliferation and progression are not fully elucidated. It is well known that abnormal glycosylation is involved in oncogenesis, and fucosylation is one of the most important types of glycosylation. In particular, fucosyltransferase 8 (FUT8) is the only FUT responsible for α1, 6-linked fucosylation (core fucosylation), and it is involved in various physiological as well as pathophysiological processes, including cancer biology. Therefore, we aimed to identify the expression of FUT8 in endometrial endometrioid carcinoma and investigate the effect of the partial silencing of the FUT8 gene on the cell proliferation of Ishikawa cells, an epithelial-like endometrial cancer cell line. Quantitative real-time PCR analysis showed that FUT8 gene expression was significantly elevated in the endometrial endometrioid carcinoma, compared to the normal endometrium. The immunostaining of FUT8 and Ulex europaeus Agglutinin 1 (UEA-1), a kind of lectin family specifically binding to fucose, was detected endometrial endometrioid carcinoma. The proliferation assay showed FUT8 partial knockdown by transfection of siRNA significantly suppressed the proliferation of Ishikawa cells, concomitant with the upregulation in the gene expressions associated with the interesting pathways associated with de-ubiquitination, aspirin trigger, mesenchymal-epithelial transition (MET) et al. It was suggested that the core fucosylation brought about by FUT8 might be involved in the proliferation of endometrial endometrioid carcinoma cells.

リンク情報
DOI
https://doi.org/10.1016/j.bbrep.2020.100740
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32099910
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7026730

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