論文

査読有り 国際誌
2020年5月8日

Advancement of capture immunoassay for real-time monitoring of hepatitis E virus-infected monkey.

Analytica chimica acta
  • Indra Memdi Khoris
  • ,
  • Ankan Dutta Chowdhury
  • ,
  • Tian-Cheng Li
  • ,
  • Tetsuro Suzuki
  • ,
  • Enoch Y Park

1110
開始ページ
64
終了ページ
71
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.aca.2020.02.020

Rapid increasing outbreak of Hepatitis E virus (HEV) shows an urgent need of HEV detection. Instead of time consuming and expensive RT-qPCR, an efficient and quick monitoring system is in utmost demand which can be comparable with the RT-qPCR in term of reliability and detection limit. An advanced platform for immunoassay has been constructed in this study by a nanozyme that constitutes anti-HEV IgG antibody-conjugated gold nanoparticles (Ab-AuNPs) as core and in situ silver deposition on the surface of Ab-AuNPs as outer shell. The virus has been entrapped on the nanocomposites while the silver-shell has decomposed back to the silver ions (Ag+) by adding a tetramethylbenzidine (TMBZ) and hydrogen peroxide (H2O2) which indirectly quantifies the target virus concentration. Counterpart to only applying nanozyme, by incorporation of the enhanced effect of Ag shell on the AuNP-based nanozyme, the advance deposition has been confirmed to prove the signal amplification mechanism in the proposed immunoassay. Most importantly, the sensor performances have examined on the HEV, collected from the HEV-infected monkey over a period of 45 days. It was successfully correlated with the standard RT-qPCR data, showing the applicability of this immunoassay as a real-time monitoring on the HEV infection. The in situ formation of AuNPs@Ag as nanozyme in this capture immunoassay leads to a promising advancement over the conventional methods and nanozyme-based immunoassay in real application which can be a good substitute of RT-qPCR in near future.

リンク情報
DOI
https://doi.org/10.1016/j.aca.2020.02.020
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32278401

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