論文

国際誌
2023年3月15日

Auxin signaling is essential for organogenesis but not for cell survival in the liverwort Marchantia polymorpha.

The Plant cell
  • Hidemasa Suzuki
  • ,
  • Hirotaka Kato
  • ,
  • Megumi Iwano
  • ,
  • Ryuichi Nishihama
  • ,
  • Takayuki Kohchi

35
3
開始ページ
1058
終了ページ
1075
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/plcell/koac367

Auxin plays pleiotropic roles in plant development via gene regulation upon its perception by the receptors TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALING F-BOX (TIR1/AFBs). This auxin-regulated transcriptional control mechanism originated in the common ancestor of land plants. Although the complete loss of TIR1/AFBs causes embryonic lethality in Arabidopsis thaliana, it is unclear whether the requirement for TIR1-mediated auxin perception in cell viability can be generalized. The model liverwort Marchantia polymorpha has a minimal auxin signaling system with only a single TIR1/AFB, MpTIR1. Here we show by genetic, biochemical, and transcriptomic analyses that MpTIR1 functions as an evolutionarily conserved auxin receptor. Null mutants and conditionally knocked-out mutants of MpTIR1 were viable but incapable of forming any organs and grew as cell masses. Principal component analysis performed using transcriptomes at various developmental stages indicated that MpTIR1 is involved in the developmental transition from spores to organized thalli, during which apical notches containing stem cells are established. In Mptir1 cells, stem cell- and differentiation-related genes were up- and downregulated, respectively. Our findings suggest that, in M. polymorpha, auxin signaling is dispensable for cell division but is essential for three-dimensional patterning of the plant body by establishing pluripotent stem cells for organogenesis, a derived trait of land plants.

リンク情報
DOI
https://doi.org/10.1093/plcell/koac367
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/36529527
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10015169
ID情報
  • DOI : 10.1093/plcell/koac367
  • PubMed ID : 36529527
  • PubMed Central 記事ID : PMC10015169

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