論文

2022年3月11日

Diminished Auxin Signaling Triggers Cellular Reprogramming by Inducing a Regeneration Factor in the Liverwort Marchantia polymorpha.

Plant & cell physiology
  • Sakiko Ishida
  • Hidemasa Suzuki
  • Aya Iwaki
  • Shogo Kawamura
  • Shohei Yamaoka
  • Mikiko Kojima
  • Yumiko Takebayashi
  • Katsushi Yamaguchi
  • Shuji Shigenobu
  • Hitoshi Sakakibara
  • Takayuki Kohchi
  • Ryuichi Nishihama
  • 全て表示

63
3
開始ページ
384
終了ページ
400
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/pcp/pcac004

Regeneration in land plants is accompanied by the establishment of new stem cells, which often involves reactivation of the cell division potential in differentiated cells. The phytohormone auxin plays pivotal roles in this process. In bryophytes, regeneration is enhanced by the removal of the apex and repressed by exogenously applied auxin, which has long been proposed as a form of apical dominance. However, the molecular basis behind these observations remains unexplored. Here, we demonstrate that in the liverwort Marchantia polymorpha, the level of endogenous auxin is transiently decreased in the cut surface of decapitated explants, and identify by transcriptome analysis a key transcription factor gene, LOW-AUXIN RESPONSIVE (MpLAXR), which is induced upon auxin reduction. Loss of MpLAXR function resulted in delayed cell cycle reactivation, and transient expression of MpLAXR was sufficient to overcome the inhibition of regeneration by exogenously applied auxin. Furthermore, ectopic expression of MpLAXR caused cell proliferation in normally quiescent tissues. Together, these data indicate that decapitation causes a reduction of auxin level at the cut surface, where, in response, MpLAXR is up-regulated to trigger cellular reprogramming. MpLAXR is an ortholog of Arabidopsis ENHANCER OF SHOOT REGENERATION 1/DORNRÖSCHEN, which has dual functions as a shoot regeneration factor and a regulator of axillary meristem initiation, the latter of which requires a low auxin level. Thus, our findings provide insights into stem cell regulation as well as apical dominance establishment in land plants.

リンク情報
DOI
https://doi.org/10.1093/pcp/pcac004
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/35001102
ID情報
  • DOI : 10.1093/pcp/pcac004
  • PubMed ID : 35001102

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