論文

査読有り
2017年12月

Adipose-derived stem cells express higher levels of type VII collagen under specific culture conditions

ARCHIVES OF DERMATOLOGICAL RESEARCH
  • Yuichiro Maeda
  • ,
  • Toshio Hasegawa
  • ,
  • Akino Wada
  • ,
  • Tatsuo Fukai
  • ,
  • Hideo Iida
  • ,
  • Atsushi Sakamoto
  • ,
  • Shigaku Ikeda

309
10
開始ページ
843
終了ページ
849
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00403-017-1781-9
出版者・発行元
SPRINGER

Type VII collagen (Col7) is a major component of the anchoring fibrils at the dermoepidermal junction. Adipose-derived stem cells (ADSCs) are a cell population highly useful in regenerative medicine because of their ease of isolation and their potential for multilineage differentiation. Based on the observations that K14 was expressed in undifferentiated ADSCs and the expression was downregulated after differentiation into adipocytes, we speculated that ADSCs are keratinocyte stem/progenitor cells. ADSCs were co-cultured with fibroblasts on type IV collagen in a medium containing all-trans retinoic acid and bone morphogenetic protein 4. At day 14 of culture in keratinocyte serum-free medium, the cells were harvested and subjected to immunofluorescence, flow cytometry, real-time PCR, and western blotting. Approximately, 45% of ADSCs were immunostained positively for anti-human cytokeratin 10, and approximately 80% were stained positively for Col7. Flow cytometry, real-time PCR, and western blotting also confirmed that differentiated ADSCs expressed higher levels of Col7. These findings support the therapeutic potential of ADSCs, not only for wound healing, but also for the correction of Col7 deficiencies.

リンク情報
DOI
https://doi.org/10.1007/s00403-017-1781-9
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28940088
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000414651400008&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00403-017-1781-9
  • ISSN : 0340-3696
  • eISSN : 1432-069X
  • PubMed ID : 28940088
  • Web of Science ID : WOS:000414651400008

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