論文

査読有り
2010年12月

A Structural Determinant for the Control of PIP2 Sensitivity in G Protein-gated Inward Rectifier K+ Channels

JOURNAL OF BIOLOGICAL CHEMISTRY
  • Atsushi Inanobe
  • ,
  • Atsushi Nakagawa
  • ,
  • Takanori Matsuura
  • ,
  • Yoshihisa Kurachi

285
49
開始ページ
38517
終了ページ
38523
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1074/jbc.M110.161703
出版者・発行元
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

Inward rectifier K+ (Kir) channels are activated by phosphatidylinositol-(4,5)-bisphosphate (PIP2), but G protein-gated Kir (K-G) channels further require either G protein beta gamma subunits (G beta gamma) or intracellular Na+ for their activation. To reveal the mechanism(s) underlying this regulation, we compared the crystal structures of the cytoplasmic domain of K-G channel subunit Kir3.2 obtained in the presence and the absence of Na+. The Na+-free Kir3.2, but not the Na+-plus Kir3.2, possessed an ionic bond connecting the N terminus and the CD loop of the C terminus. Functional analyses revealed that the ionic bond between His-69 on the N terminus and Asp-228 on the CD loop, which are known to be critically involved in G beta gamma- and Na+-dependent activation, lowered PIP2 sensitivity. The conservation of these residues within the K-G channel family indicates that the ionic bond is a character that maintains the channels in a closed state by controlling the PIP2 sensitivity.

Web of Science ® 被引用回数 : 16

リンク情報
DOI
https://doi.org/10.1074/jbc.M110.161703
CiNii Articles
http://ci.nii.ac.jp/naid/80021481980
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/20880843
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000284625600057&DestApp=WOS_CPL

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