論文

査読有り
2016年9月

Zebrafish lines expressing UAS-driven red probes for monitoring cytoskeletal dynamics

GENESIS
  • Takamasa Mizoguchi
  • ,
  • Koichi Kawakami
  • ,
  • Motoyuki Itoh

54
9
開始ページ
483
終了ページ
489
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/dvg.22955
出版者・発行元
WILEY-BLACKWELL

Actin filaments and microtubules are principal components of the cytoskeleton that regulate the basic cellular phenomena underlying many fundamental cellular processes. Therefore, analyzing their dynamics in living cells is important for understanding cellular events more precisely. In this article, we report two novel transgenic zebrafish lines expressing red fluorescent proteins tagged with Lifeact or EB1 that interact with actin filaments and microtubule plus ends, respectively, under the control of the GAL4-UAS system. Using these transgenic lines, we could detect F-actin and microtubule plus end dynamics in specific tissues of living zebrafish embryos by crossing with GAL4 driver lines. In addition, we could achieve multi-color imaging using these transgenic lines with GFP-expressing transgenic lines. Therefore, our transgenic lines that carry UAS-driven red fluorescent cytoskeletal probes are useful tools for analyzing spatiotemporal changes of the cytoskeletal elements using multicolor live imaging.

Web of Science ® 被引用回数 : 3

リンク情報
DOI
https://doi.org/10.1002/dvg.22955
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000383583100003&DestApp=WOS_CPL
URL
http://www.scopus.com/inward/record.url?eid=2-s2.0-84986593138&partnerID=MN8TOARS
URL
http://orcid.org/0000-0002-9475-6495

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