2009年10月6日
48.抗BRI1抗体のスクリーニングとその応用(口頭発表)
植物化学調節学会研究発表記録集
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- 巻
- 号
- 44
- 開始ページ
- 62
- 終了ページ
- 62
- 記述言語
- 日本語
- 掲載種別
- 出版者・発行元
- 植物化学調節学会
Immunomodulation is a means to modulate an organism's function by antibody production to capture either endogenous or exogenous antigens. We screened single chain antibodies (scFvs) against the extracellular domain of BRI1 (BRI1-LRR), which is known as the binding domain of brassinosteroids, from phage display scFv libraries in order to create plants in which brassinosteroid signaling is impaired by immunomodulation. BRI1-LRR was produced as a fusion protein with GST, and was fixed either on immunotubes by adsorption or on glutathione-Sepharose, against which phages expressing different scFvs, J-1 and J-2, were respectively selected. J-1 showed binding activity to GST-BRI1-LRR fusion protein with clearly lower affinity to GST in ELISA, while J-2 showed higher specificity to GST-BRI1-LRR. In Western blot analysis, J-1, but not J-2, could detect GST-BRI1-LRR, suggesting that J-2 recognizes an epitope formed as a three-dimensional structure of BRI1-LRR.
- リンク情報
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- CiNii Articles
- http://ci.nii.ac.jp/naid/110007468308
- CiNii Books
- http://ci.nii.ac.jp/ncid/AN10348274
- ID情報
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- ISSN : 0919-1887
- CiNii Articles ID : 110007468308
- CiNii Books ID : AN10348274