論文

査読有り
2016年12月

Rice CYO1, an ortholog of Arabidopsis thaliana cotyledon chloroplastbiogenesis factor AtCYO1, is expressed in leaves and involved in photosynthetic performance

JOURNAL OF PLANT PHYSIOLOGY
  • Jun Tominaga
  • ,
  • Haruka Mizutani
  • ,
  • Daisuke Horikawa
  • ,
  • Yasutoshi Nakahara
  • ,
  • Tsuneaki Takami
  • ,
  • Wataru Sakamoto
  • ,
  • Atsushi Sakamoto
  • ,
  • Hiroshi Shimada

207
開始ページ
78
終了ページ
83
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jplph.2016.10.005
出版者・発行元
ELSEVIER GMBH, URBAN & FISCHER VERLAG

In the dicotyledonous plant Arabidopsis thaliana, the cotyledon chloroplast biogenesis factor AtCYO1 is crucial for the biogenesis of cotyledon chloroplasts. Arabidopsis mutants lacking AtCYO1 have pale cotyledons but develop normal mature leaves. In the monocotyledonous plant Oryza sativa, the gene OsCYO1 has high sequence identity to AtCYO1, but its function is unknown. We examined the role of OsCYO1 in O. sativa. We first confirmed that transformation with OsCYO1 could recover the phenotype of the Arabidopsis cyo1 mutant. Similar to AtCYO1, recombinant OsCYO1 has protein disulfide reductase (PDR) activity, which increased as a function of dieosin glutathione disulfide concentration with an apparent K-m of 3.2 mu M and K-cat of 0.53 min(-1). The PDR activity was reduced when NADPH or NADH was used as an electron donor; however, PDR activity was observed with OsCYO1 and glutathione, suggesting that glutathione may serve as a reducing agent for OsCYO1 in vivo. In O. sativa, the OsCYO1 transcript level was higher in leaves compared with the coleoptile, which is the first leaf-like organ that forms during rice embryogenesis. Many OsCYO1 mutant lines defective in RNA interference had green leaves, however, three mutant lines had not only albino coleoptile but also albino leaves. Those having green leaves reduced photosynthetic performance in leaves. Our results demonstrate that OsCYO1 is enzymatically equivalent to AtCYO1 but that the physiological role of OsCYO1 in monocotyledonous plants may differ from that of AtCYO1 in dicotyledonous plants. (C) 2016 Elsevier GmbH. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.jplph.2016.10.005
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000389596600009&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.jplph.2016.10.005
  • ISSN : 0176-1617
  • eISSN : 1618-1328
  • Web of Science ID : WOS:000389596600009

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