論文

査読有り
2011年11月

Detection of the Onset of Ischemia and Carcinogenesis by Hypoxia-Inducible Transcription Factor-Based In Vivo Bioluminescence Imaging

PLOS ONE
  • Tetsuya Kadonosono
  • Takahiro Kuchimaru
  • Shuichi Yamada
  • Yumi Takahashi
  • Atsushi Murakami
  • Taeko Tani
  • Hitomi Watanabe
  • Tomoharu Tanaka
  • Kiichi Hirota
  • Masahiro Inoue
  • Tetsuya Tsukamoto
  • Takeshi Toyoda
  • Koji Urano
  • Kazuhiko Machida
  • Tomoo Eto
  • Tomoyuki Ogura
  • Hideki Tsutsumi
  • Mamoru Ito
  • Masahiro Hiraoka
  • Gen Kondoh
  • Shinae Kizaka-Kondoh
  • 全て表示

6
11
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0026640
出版者・発行元
PUBLIC LIBRARY SCIENCE

An animal model for the early detection of common fatal diseases such as ischemic diseases and cancer is desirable for the development of new drugs and treatment strategies. Hypoxia-inducible factor 1 (HIF-1) is a transcription factor that regulates oxygen homeostasis and plays key roles in a number of diseases, including cancer. Here, we established transgenic (Tg) mice that carry HRE/ODD-luciferase (HOL) gene, which generates bioluminescence in an HIF-1-dependent manner and was successfully used in this study to monitor HIF-1 activity in ischemic tissues. To monitor carcinogenesis in vivo, we mated HOL mice with rasH2 Tg mice, which are highly sensitive to carcinogens and are used for short-term carcinogenicity assessments. After rasH2-HOL Tg mice were treated with N-methyl-N-nitrosourea, bioluminescence was detected noninvasively as early as 9 weeks in tissues that contained papillomas and malignant lesions. These results suggest that the Tg mouse lines we established hold significant potential for monitoring the early onset of both ischemia and carcinogenesis and that these lines will be useful for screening chemicals for carcinogenic potential.

リンク情報
DOI
https://doi.org/10.1371/journal.pone.0026640
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000297348800008&DestApp=WOS_CPL
ID情報
  • DOI : 10.1371/journal.pone.0026640
  • ISSN : 1932-6203
  • Web of Science ID : WOS:000297348800008

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