Takahiro Masuya

Profile Information

Affiliation: Graduate School of Agriculture, Division of Applied Life Sciences, Kyoto University

Degree: 博士（農学）(京都大学)

ORCID ID: https://orcid.org/0000-0003-4950-0527
J-GLOBAL ID: 201801009684484295
researchmap Member ID: B000347809

Research Interests

Research Areas

Life sciences / Bioorganic chemistry /

Research History

Nov, 2021 - Present

Assistant Professor, Graduate School of Agriculture, Kyoto University
Dec, 2018 - Oct, 2021

Graduate School of Agriculture, Kyoto University
Apr, 2017 - Nov, 2018

School of Pharmaceutical Sciences, University of Shizuoka
Apr, 2015 - Mar, 2017

日本学術振興会特別研究員(DC2)

Education

Apr, 2014 - Mar, 2017

Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Apr, 2012 - Mar, 2014

Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Apr, 2008 - Mar, 2012

Faculty of Agriculture, Kyoto University

Committee Memberships

Apr, 2021 - Present

農薬デザイン研究会委員, 日本農薬学会

Papers

Respiratory complex I in mitochondrial membrane catalyzes oversized ubiquinones

Ryo Ikunishi, Ryohei Otani, Takahiro Masuya, Kyoko Shinzawa-Itoh, Tomoo Shiba, Masatoshi Murai, Hideto Miyoshi

Journal of Biological Chemistry, 105001-105001, Jun, 2023
Discovery of Bis-sulfonamides as Novel Inhibitors of Mitochondrial NADH-Quinone Oxidoreductase (Complex I)

Atsuhito Tsuji, Takahiro Masuya, Norihito Arichi, Shinsuke Inuki, Masatoshi Murai, Hideto Miyoshi, Hiroaki Ohno

ACS Medicinal Chemistry Letters, 14(2) 211-216, Jan, 2023
Cryo-EM structures of Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae

Jun-ichi Kishikawa, Moe Ishikawa, Takahiro Masuya, Masatoshi Murai, Yuki Kitazumi, Nicole L. Butler, Takayuki Kato, Blanca Barquera, Hideto Miyoshi

Nature Communications, 13(1), Jul, 2022
The side chain of ubiquinone plays a critical role in Na+ translocation by the NADH-ubiquinone oxidoreductase (Na+-NQR) from Vibrio cholerae

Moe Ishikawa, Takahiro Masuya, Seina Kuroda, Shinpei Uno, Nicole L. Butler, Sara Foreman, Masatoshi Murai, Blanca Barquera, Hideto Miyoshi

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1863(5) 148547-148547, Jun, 2022
Diverse reaction behaviors of artificial ubiquinones in mitochondrial respiratory complex I

Shinpei Uno, Takahiro Masuya, Oleksii Zdorevskyi, Ryou Ikunishi, Kyoko Shinzawa-Itoh, Jonathan Lasham, Vivek Sharma, Masatoshi Murai, Hideto Miyoshi

Journal of Biological Chemistry, 102075-102075, May, 2022
Comprehensive understanding of multiple actions of anticancer drug tamoxifen in isolated mitochondria

Yufu Unten, Masatoshi Murai, Tomoki Koshitaka, Kotaro Kitao, Osamu Shirai, Takahiro Masuya, Hideto Miyoshi

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1863(2) 148520-148520, Feb, 2022
Natural tetramic acids elicit multiple inhibitory actions against mitochondrial machineries presiding over oxidative phosphorylation

Yufu Unten, Masatoshi Murai, Katsuyuki Sakai, Yukihiro Asami, Takenori Yamamoto, Takahiro Masuya, Hideto Miyoshi

Bioscience, Biotechnology, and Biochemistry, Oct, 2021 Peer-reviewed
Specific chemical modification explores dynamic structure of the NqrB subunit in Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae

Moe Ishikawa, Takahiro Masuya, Hinako Tanaka, Wataru Aoki, Noam Hantman, Nicole L. Butler, Masatoshi Murai, Blanca Barquera, Hideto Miyoshi

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1862(8) 148432-148432, Aug, 2021 Peer-reviewed
Pinpoint Dual Chemical Cross-Linking Explores the Structural Dynamics of the Ubiquinone Reaction Site in Mitochondrial Complex I

Takahiro Masuya, Shinpei Uno, Masatoshi Murai, Hideto Miyoshi

Biochemistry, 60(10) 813-824, Mar, 2021 Peer-reviewed
Uncovering hidden sesquiterpene biosynthetic pathway through expression boost area-mediated productivity enhancement in basidiomycete

Shihori Asai, Yuta Tsunematsu, Takahiro Masuya, Junnosuke Otaka, Hiroyuki Osada, Kenji Watanabe

The Journal of Antibiotics, 73(10) 721-728, Oct, 2020 Peer-reviewed
Inhibitors of a Na+-pumping NADH-ubiquinone oxidoreductase play multiple roles to block enzyme function

Takahiro Masuya, Yuki Sano, Hinako Tanaka, Nicole L. Butler, Takeshi Ito, Tatsuhiko Tosaki, Joel E. Morgan, Masatoshi Murai, Blanca Barquera, Hideto Miyoshi

Journal of Biological Chemistry, 295(36) 12739-12754, Sep, 2020 Peer-reviewed
IACS-010759, a potent inhibitor of glycolysis-deficient hypoxic tumor cells, inhibits mitochondrial respiratory complex I through a unique mechanism

Atsuhito Tsuji, Takumi Akao, Takahiro Masuya, Masatoshi Murai, Hideto Miyoshi

Journal of Biological Chemistry, 295(21) 7481-7491, May, 2020 Peer-reviewed
Oversized ubiquinones as molecular probes for structural dynamics of the ubiquinone reaction site in mitochondrial respiratory complex I

Shinpei Uno, Takahiro Masuya, Kyoko Shinzawa-Itoh, Jonathan Lasham, Outi Haapanen, Tomoo Shiba, Daniel Ken Inaoka, Vivek Sharma, Masatoshi Murai, Hideto Miyoshi

Journal of Biological Chemistry, 295(8) 2449-2463, Feb, 2020 Peer-reviewed
Genomic Mushroom Hunting Decrypts Coprinoferrin, A Siderophore Secondary Metabolite Vital to Fungal Cell Development

Yuta Tsunematsu, Jun Takanishi, Shihori Asai, Takahiro Masuya, Takehito Nakazawa, Kenji Watanabe

Organic Letters, 21(18) 7582-7586, Sep, 2019 Peer-reviewed
Biosynthesis of lagopodins in mushroom involves a complex network of oxidation reactions

Takahiro Masuya, Yuta Tsunematsu, Yuichiro Hirayama, Michio Sato, Hiroshi Noguchi, Takehito Nakazawa, Kenji Watanabe

Organic & Biomolecular Chemistry, 17(2) 234-239, Jan, 2019 Peer-reviewed
Pinpoint Chemical Modification of the Quinone-Access Channel of Mitochondrial Complex I via a Two-Step Conjugation Reaction

Takahiro Masuya, Masatoshi Murai, Takeshi Ito, Shunsuke Aburaya, Wataru Aoki, Hideto Miyoshi

BIOCHEMISTRY, 56(32) 4279-4287, Aug, 2017 Peer-reviewed
Characterization of the reaction of decoupling ubiquinone with bovine mitochondrial respiratory complex I

Takahiro Masuya, Kenji Okuda, Masatoshi Murai, Hideto Miyoshi

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 80(8) 1464-1469, Aug, 2016 Peer-reviewed
Specific Methylation of Asp160 (49 kDa subunit) Located inside the Quinone Binding Cavity of Bovine Mitochondrial Complex I

Masatoshi Murai, Hiroyuki Inaoka, Takahiro Masuya, Shunsuke Aburaya, Wataru Aoki, Hideto Miyoshi

BIOCHEMISTRY, 55(23) 3189-3197, Jun, 2016 Peer-reviewed
Pinpoint Chemical Modification of Asp160 in the 49 kDa Subunit of Bovine Mitochondrial Complex I via a Combination of Ligand-Directed Tosyl Chemistry and Click Chemistry

Takahiro Masuya, Masatoshi Mural, Hironobu Morisaka, Hideto Miyoshi

BIOCHEMISTRY, 53(49) 7816-7823, Dec, 2014 Peer-reviewed
Site-Specific Chemical Labeling of Mitochondrial Respiratory Complex I through Ligand-Directed Tosylate Chemistry

Takahiro Masuya, Masatoshi Murai, Kentaro Ifuku, Hironobu Morisaka, Hideto Miyoshi

BIOCHEMISTRY, 53(14) 2307-2317, Apr, 2014 Peer-reviewed

Presentations

ミトコンドリア呼吸鎖複合体-Iを阻害するアセトゲニンの結合部位の同定

西田美咲, 桝谷貴洋, 村井正俊, 三芳秀人

日本農芸化学会2023年度大会, Mar, 2023
クライオ電子顕微鏡によるコレラ菌Na+輸送性NADH-ユビキノン酸化還元酵素の構造解明

石川萌, 岸川淳一, 桝谷貴洋, 村井正俊, 北隅優希, Nicole L. Butler, 加藤貴之, Blanca Barquera, 三芳秀人

日本農芸化学会2023年度大会, Mar, 2023
人工キノン類を用いた呼吸鎖複合体-Iのキノン結合ポケットに関する研究

生西凌, 大谷燎平, 桝谷貴洋, 村井正俊, 三芳秀人

日本農芸化学会2023年度大会, Mar, 2023
ミトコンドリア呼吸鎖複合体-Iにおける脱共役ユビキノン類の作用機構

越高知生, 富澤康平, 桝谷貴洋, 村井正俊, 三芳秀人

日本農芸化学会2023年度大会, Mar, 2023
コレラ菌Na+輸送性NADH－ユビキノン酸化還元酵素におけるユビキノン側鎖の結合部位の同定

石川萌, 桝谷貴洋, 村井正俊, Nicole L. Butler, Blanca Barquera, 三芳秀人

日本農薬学会第48回大会, Mar, 2023
ミトコンドリア呼吸鎖複合体-I を阻害するアセトゲニンの結合部位の同定

西田美咲, 桝谷貴洋, 村井正俊, 三芳秀人

日本農薬学会第48回大会, Mar, 2023
ミトコンドリア呼吸鎖複合体-I のケミカルバイオロジー

桝谷貴洋

第26回農薬相模セミナー, Jan, 2023 Invited
人工キノン類を用いたミトコンドリア呼吸鎖複合体-Iのユビキノン反応部位の特性解明

桝谷貴洋, 宇野晋平, 越高知生, 生西凌, 富澤康平, 大谷燎平, 奥田健司, 村井正俊, 三芳秀人

日本生体エネルギー研究会第48回討論会, Dec, 2022
コレラ菌Na+輸送性NADH-ユビキノン酸化還元酵素におけるユビキノン側鎖の結合部位の同定

石川萌, 桝谷貴洋, 村井正俊, Nicole L. Butler, Blanca Barquera, 三芳秀人

日本生体エネルギー研究会第48回討論会, Dec, 2022
コレラ菌Na+輸送性NADH-キノン酸化還元酵素に対する阻害剤の作用機構

石川萌, 岸川淳一, 桝谷貴洋, 村井正俊, 北隅優希, Nicole L. Butler, 加藤貴之, Blanca Barquera, 三芳秀人

第37回農薬デザイン研究会, Nov, 2022
クライオ電子顕微鏡を用いたコレラ菌 Na+輸送性 NADH-ユビキノン酸化還元酵素の構造解明

石川萌, 岸川淳一, 桝谷貴洋, 村井正俊, 北隅優希, Nicole L. Butler, 加藤貴之, Blanca Barquera, 三芳秀人

第60回日本生物物理学会年会, Sep, 2022
コレラ菌Na+輸送型NADH-キノン酸化還元酵素の阻害剤コロルミシンの作用機序

桝谷貴洋, 石川萌, 田中比奈子, 岸川淳一, Blanca Barquera, 北隅優希, 村井正俊, 三芳秀人

第64回天然有機化合物討論会, Sep, 2022
コレラ菌NADH-ユビキノン酸化還元酵素のNa+輸送におけるユビキノン側鎖の重要性

石川萌, 桝谷貴洋, 黒田聖奈, 宇野晋平, 志波智生, 稲岡健ダニエル, 村井正俊, Blanca Barquera, 三芳秀人

第47回日本農薬学会大会, Mar, 2022
光親和性ユビキノン類を用いた呼吸鎖複合体-I のキノン反応ポケットの構造特性の解明

宇野晋平, 生西凌, 桝谷貴洋, 伊藤(新澤) 恭子, 村井正俊, 三芳秀人

第47回日本農薬学会大会, Mar, 2022
コレラ菌NADH-ユビキノン酸化還元酵素のNa+輸送におけるユビキノン側鎖の重要性

石川萌, 桝谷貴洋, 黒田聖奈, 宇野晋平, 志波智生, 稲岡健ダニエル, 村井正俊, Blanca Barquera, 三芳秀人

日本農芸化学会2022年度大会, Mar, 2022
コレラ菌Na+-輸送型NADH-ユビキノン酸化還元酵素の位置特異的化学修飾

田中比奈子, 石川萌, 桝谷貴洋, 青木航, 村井正俊, Blanca Barquera, 三芳秀人

日本農芸化学会2022年度大会, Mar, 2022
合成ユビキノンプローブを用いた出芽酵母のユビキノン取り込みに関与するタンパク質の探索

黒田聖奈, 村井正俊, 桝谷貴洋, 奥公秀, 三芳秀人

日本農芸化学会2022年度大会, Mar, 2022
抗がん剤タモキシフェンのラット肝臓ミトコンドリアにおける作用機構研究

運天優歩, 村井正俊, 越高知生, 北尾虎太郎, 白井理, 桝谷貴洋, 三芳秀人

日本農芸化学会2022年度大会, Mar, 2022
ミトコンドリア呼吸鎖複合体-Iにおける人工ユビキノン類の多様な反応機構

宇野晋平, 生西凌, 桝谷貴洋, 伊藤(新澤) 恭子, 村井正俊, 三芳秀人

日本農芸化学会2022年度大会, Mar, 2022
抗がん剤タモキシフェンのラット肝臓ミトコンドリアにおける作用機構研究

運天優歩, 村井正俊, 越高知生, 北尾虎太郎, 白井理, 桝谷貴洋, 三芳秀人

日本生体エネルギー研究会第47回討論会, Dec, 2021

Research Projects

Elucidation of sodium translocation mechanism of Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae

Grants-in-Aid for Scientific Research, Grant-in-Aid for Early-Career Scientists, Japan Society for the Promotion of Science, Apr, 2022 - Mar, 2024
Inhibition mechanism of IACS-010759 on mitochondrial respiratory complex I, a potent inhibitor of hypoxic tumor cells

Grants-in-Aid for Scientific Research, Grant-in-Aid for Early-Career Scientists, Japan Society for the Promotion of Science, Apr, 2020 - Mar, 2022

Masuya Takahrio
呼吸鎖複合体－Ｉの１分子計測を目指したプローブ分子の創製と特異的化学修飾法の確立

科学研究費助成事業, 特別研究員奨励費, 日本学術振興会, Apr, 2015 - Mar, 2017

桝谷貴洋

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