MISC

国際誌
2015年6月

GM130 is a parallel tetramer with a flexible rod-like structure and N-terminally open (Y-shaped) and closed (I-shaped) conformations.

The FEBS journal
  • Ryuichi Ishida
  • ,
  • Akitsugu Yamamoto
  • ,
  • Kazuhisa Nakayama
  • ,
  • Miwa Sohda
  • ,
  • Yoshio Misumi
  • ,
  • Takuo Yasunaga
  • ,
  • Nobuhiro Nakamura

282
11
開始ページ
2232
終了ページ
44
記述言語
英語
掲載種別
DOI
10.1111/febs.13271
出版者・発行元
WILEY-BLACKWELL

GM130 is a cytoplasmic peripheral membrane protein localized on the cis side of the Golgi apparatus. GM130 is proposed to function as a membrane skeleton, maintaining the structure of the Golgi apparatus, and as a vesicle tether that facilitates vesicle fusion to the Golgi membrane. More than 60% of the GM130 molecule is believed to exist as coiled-coil structures with a probability above 90%, based on its primary amino acid sequence. The predicted coiled-coil region was similar to that of yeast Uso1p and its mammalian homolog, p115, both of which form coiled-coil homodimers. Therefore, GM130 has long been thought to form a homodimer with a rod-like shape. However, our biochemical and electron microscopical analyses revealed that GM130 is a parallel homotetramer with a flexible rod-like structure with I- and Y-shaped conformations. The structure of the N-terminal region may interchange between an open conformation (branched or Y-shaped) and a closed conformation (non-branched or I-shaped), possibly with the help of interacting molecules. This conformational change may alter the oligomeric state of the GM130 molecules and the function of GM130 in the vesicle tethering and the maintenance of the Golgi structure.

リンク情報
DOI
https://doi.org/10.1111/febs.13271
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25787021
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000355664000013&DestApp=WOS_CPL
URL
http://www.scopus.com/inward/record.url?eid=2-s2.0-84927144686&partnerID=MN8TOARS
ID情報
  • DOI : 10.1111/febs.13271
  • ISSN : 1742-464X
  • eISSN : 1742-4658
  • ORCIDのPut Code : 28086809
  • PubMed ID : 25787021
  • SCOPUS ID : 84927144686
  • Web of Science ID : WOS:000355664000013

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