論文

国際誌
2017年3月17日

PCGF6-PRC1 suppresses premature differentiation of mouse embryonic stem cells by regulating germ cell-related genes.

eLife
  • Mitsuhiro Endoh
  • Takaho A Endo
  • Jun Shinga
  • Katsuhiko Hayashi
  • Anca Farcas
  • Kit-Wan Ma
  • Shinsuke Ito
  • Jafar Sharif
  • Tamie Endoh
  • Naoko Onaga
  • Manabu Nakayama
  • Tomoyuki Ishikura
  • Osamu Masui
  • Benedikt M Kessler
  • Toshio Suda
  • Osamu Ohara
  • Akihiko Okuda
  • Robert Klose
  • Haruhiko Koseki
  • 全て表示

6
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.7554/eLife.21064

The ring finger protein PCGF6 (polycomb group ring finger 6) interacts with RING1A/B and E2F6 associated factors to form a non-canonical PRC1 (polycomb repressive complex 1) known as PCGF6-PRC1. Here, we demonstrate that PCGF6-PRC1 plays a role in repressing a subset of PRC1 target genes by recruiting RING1B and mediating downstream mono-ubiquitination of histone H2A. PCGF6-PRC1 bound loci are highly enriched for promoters of germ cell-related genes in mouse embryonic stem cells (ESCs). Conditional ablation of Pcgf6 in ESCs leads to robust de-repression of such germ cell-related genes, in turn affecting cell growth and viability. We also find a role for PCGF6 in pre- and peri-implantation mouse embryonic development. We further show that a heterodimer of the transcription factors MAX and MGA recruits PCGF6 to target loci. PCGF6 thus links sequence specific target recognition by the MAX/MGA complex to PRC1-dependent transcriptional silencing of germ cell-specific genes in pluripotent stem cells.

リンク情報
DOI
https://doi.org/10.7554/eLife.21064
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28304275
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5375644
ID情報
  • DOI : 10.7554/eLife.21064
  • PubMed ID : 28304275
  • PubMed Central 記事ID : PMC5375644

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