The culture behavior of the osteoblast-like cells on the calcium phosphate (CP) films interacted with phospholipid vesicle (PV) were investigated. The different CP crystalline phases were prepared from the biological solutions such as simulated body fluid (SBF) and unique SBF, which were called as CP/PV and U-CP/PV films, respectively, and these were used after the sterilization. The protein adsorption amounts on the CP/PV films were higher than that of the PV film. The osteoblast-like cells cultured on the U-CP/PV film had the longest length along with the extension direction. The density of the osteoblast-like cells adhered on the CP/PV and U-CP/PV films were higher, suggesting the preferential adhesion on the surfaces. The differentiated osteoblasts on the films were visualized by the alkaline phosphatase and Alizarin-red-S staining methods. It was suggested that the osteoblasts cultured on the CP/PV and U-CP/PV films exhibited the possibility of the high osteogenic activities. Therefore, the PV-interacted CP films prepared from the biological solutions are useful for the osteoblast culture plates.