2014年12月
A Fine-Scale Phylogenetic Analysis of Free-Living Burkholderia Species in Sugarcane Field Soil
MICROBES AND ENVIRONMENTS
- 巻
- 29
- 号
- 4
- 開始ページ
- 434
- 終了ページ
- 437
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1264/jsme2.ME14122
- 出版者・発行元
- JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
The diversity and abundance of Burkholderia species in sugarcane field soils were investigated by a 16S rRNA gene-based approach using genus-specific primers. A total of 365,721 sequences generated by the Illumina MiSeq platform were assigned to the genus Burkholderia. Nearly 58% of these sequences were placed in a previously defined cluster, including stinkbug symbionts. Quantitative PCR analysis revealed a consistent number of 16S rRNA gene copies for Burkholderia species (10(7) g(-1) soil) across the sampled fields. C/N, pH, and nitrate concentrations were important factors shaping the Burkholderia community structure; however, their impacts were not significant considering the overall genus size.
- リンク情報
- ID情報
-
- DOI : 10.1264/jsme2.ME14122
- ISSN : 1342-6311
- Web of Science ID : WOS:000346734500016