論文

2017年11月10日

The anti-inflammatory pathway regulated via nicotinic acetylcholine receptors in rat intestinal mesothelial cells.

The Journal of veterinary medical science
  • Taiki Mihara
  • ,
  • Wataru Otsubo
  • ,
  • Kazuhide Horiguchi
  • ,
  • Shoma Mikawa
  • ,
  • Noriyuki Kaji
  • ,
  • Satoshi Iino
  • ,
  • Hiroshi Ozaki
  • ,
  • Masatoshi Hori

79
11
開始ページ
1795
終了ページ
1802
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1292/jvms.17-0304

Regulation of inflammation in intestinal mesothelial cells in the abdominal cavity is important for the pathogeny of clinical conditions, such as postoperative ileus, peritonitis and encapsulating peritoneal sclerosis. Here we have examined the inflammatory effect of lipopolysaccharide (LPS) and the anti-inflammatory effect of nicotinic acetylcholine receptor stimulation in rat intestinal mesothelial cells. LPS upregulated mRNA expression of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), monocyte chemotactic protein-1 (MCP-1) and inducible nitric oxide synthase (iNOS). The α7, α9 and α10 subunits of nicotinic acetylcholine receptor were detected in intestinal mesothelial cells. Nicotine (10 nM) significantly inhibited LPS-induced mRNA expression of IL-1β and iNOS, but not TNF-α and MCP-1. In addition, the α7 nicotinic acetylcholine receptor selective agonist, PNU-282987 (10 nM), significantly inhibited LPS-induced mRNA expression of IL-1β but not TNF-α, iNOS and MCP-1. Finally, we found that enteric nerves adhered to intestinal mesothelial cells located under the ileal serosa. In conclusion, intestinal mesothelial cells react to LPS to induce the production of nitric oxide from iNOS. The anti-inflammatory action of intestinal mesothelial cells expressing α7nAChR may be mediated via their connectivity with enteric nerves.

リンク情報
DOI
https://doi.org/10.1292/jvms.17-0304
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28931778
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5709555
ID情報
  • DOI : 10.1292/jvms.17-0304
  • PubMed ID : 28931778
  • PubMed Central 記事ID : PMC5709555

エクスポート
BibTeX RIS