2017年11月21日
Integration of Au nano-pillars and SAM enables protein patterning with designed spacing at single molecule level
2017 IEEE 17th International Conference on Nanotechnology, NANO 2017
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- 開始ページ
- 311
- 終了ページ
- 314
- 記述言語
- 英語
- 掲載種別
- 研究論文(国際会議プロシーディングス)
- DOI
- 10.1109/NANO.2017.8117281
- 出版者・発行元
- Institute of Electrical and Electronics Engineers Inc.
In cells, motor proteins do not work alone but rather move in ensemble when they support several cellular functions including mitosis and beating of flagellum. Until now, collective transportation by motors was poorly understood due to the experimental difficulty in controlling the number and arrangement of motors, which are considered to affect the transportation. In this report, we propose a novel nano-patterning method to control the number of kinesin molecules and the spacing between molecules, which transport a single microtubule filament. We fabricated Au nano-pillar array on Si/SiO2 substrates. The SiO2 surface was coated with a silane-poly(ethylene glycol) (PEG) self-assembled monolayer (SAM) using a silane-coupling reaction to eliminate non-specific adsorption of kinesin molecules. By designing the arrangement of pillars, the number of kinesin molecules and the spacing between molecules can be defined, and their effects on kinesin motility was investigated. Two motor species with different processivity, kinesin-1 and Ncd, were patterned individually. We found the number of motors affected the microtubule velocity in the case of non-processive Ncd, but not in the case of processive kinesin-1.
- ID情報
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- DOI : 10.1109/NANO.2017.8117281
- SCOPUS ID : 85041193977