2001年
Diverse transcriptional initiation revealed by fine, large-scale mapping of mRNA start sites
EMBO Reports
- 巻
- 2
- 号
- 5
- 開始ページ
- 388
- 終了ページ
- 393
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/embo-reports/kve085
Determination of the mRNA start site is the first step in identifying the promoter region, which is of key importance for transcriptional regulation of gene expression. The 'oligo-capping' method enabled us to introduce a sequence tag to the first base of an mRNA by replacing the cap structure of the mRNA. Using cDNA libraries made from oligo-capped mRNAs, we could identify the transcriptional start site of an individual mRNA just by sequencing the 5′-end of the cDNA. The fine mapping of transcriptional start sites was performed for 5880 mRNAs in 276 human genes. Contrary to our expectations, the majority of the genes showed a diverse distribution of transcriptional start sites. They were distributed over 61.7 bp with a standard deviation of 19.5. Our finding may reflect the dynamic nature of transcriptional initiation events of human genes in vivo.
- リンク情報
- ID情報
-
- DOI : 10.1093/embo-reports/kve085
- ISSN : 1469-221X
- PubMed ID : 11375929
- SCOPUS ID : 0034991911