論文

査読有り 責任著者
2009年8月

Inhibition of nucleation and growth of ice by poly(vinyl alcohol) in vitrification solution

CRYOBIOLOGY
  • Hai-Yan Wang
  • ,
  • Takaaki Inada
  • ,
  • Kunio Funakoshi
  • ,
  • Shu-Shen Lu

59
1
開始ページ
83
終了ページ
89
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.cryobiol.2009.04.013
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Control of ice formation is crucial in cryopreservation of biological substances. Successful vitrification using several additives that inhibit ice nucleation in vitrification solutions has previously been reported. Among these additives, here we focused on a synthetic polymer, poly(vinyl alcohol) (PVA), and investigated the effects of PVA on nucleation and growth of ice in 35% (w/w) aqueous 1,2-propanediol solution by using a differential scanning calorimetry (DSC) system equipped with a cryomicroscope. First, the freezing temperature of the solution was measured using the DSC system, and then the change in ice fraction in the solution during cooling was evaluated based on images obtained using the cryomicroscope, at different concentrations of PVA between 0% and 3% (w/w). Based on the ice fraction, the change in residual solution concentration during cooling was also evaluated and then plotted on the state diagram of aqueous 1,2-propanediol solution. Results indicated that, when the partially glassy and partially frozen state was intentionally allowed, the addition of PVA effectively inhibited not only ice nucleation but also ice growth in the vitrification solution. The effect of PVA on ice growth in the vitrification solution was explained based on kinetic limitations mainly due to mass transport. The interfacial kinetics also might limit ice growth in the vitrification solution only when the ice growth rate decreased below a critical value. This coincides with the fact that PVA exhibits a unique antifreeze activity in the same manner as antifreeze proteins when ice growth rate is lower than a critical value. (C) 2009 Elsevier Inc. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.cryobiol.2009.04.013
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000268059000014&DestApp=WOS_CPL
URL
https://publons.com/publon/8201538/
ID情報
  • DOI : 10.1016/j.cryobiol.2009.04.013
  • ISSN : 0011-2240
  • eISSN : 1090-2392
  • ORCIDのPut Code : 71581785
  • Web of Science ID : WOS:000268059000014

エクスポート
BibTeX RIS