論文

査読有り
2019年9月

Electro-osmotic trapping and compression of single DNA molecules while passing through a nanopore

ANALYST
  • Hirohito Yamazaki
  • ,
  • Takaha Mizuguchi
  • ,
  • Keiko Esashika
  • ,
  • Toshiharu Saiki

144
18
開始ページ
5381
終了ページ
5388
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1039/c9an01253b
出版者・発行元
ROYAL SOC CHEMISTRY

Complicated DNA molecular behaviors exist during translocation into a nanopore because their large and coiled structure needs to unwind. In this work, we investigated DNA translocation dynamics through a 200 nm pore using a fast photon counting system (FPCS). We found that the dwell time of the DNA molecules depends on the inverse of voltage (tau proportional to V-1.02) with a large constant term (similar to 1 ms). In other words, spherical fluorescence bead translocation involves electrophoresis as well as other additional factors. Our theoretical calculation suggested that one additional factor is electro-osmotic trapping associated with the instantaneous Brownian motion before and after translocation. Furthermore, compressed DNA molecular conformation was seen as a result of the increase of peak photon counts and the decrease of electrophoretic mobility with voltage. Our experiments showed that the polymers at the vicinity of a nanopore can be trapped and compressed, which is necessary to understand how to control the polymer translocation into a nanopore.

Web of Science ® 被引用回数 : 3

リンク情報
DOI
https://doi.org/10.1039/c9an01253b
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000484974900006&DestApp=WOS_CPL
ID情報
  • DOI : 10.1039/c9an01253b
  • ISSN : 0003-2654
  • eISSN : 1364-5528
  • Web of Science ID : WOS:000484974900006

エクスポート
BibTeX RIS