- ROYAL SOC CHEMISTRY
Complicated DNA molecular behaviors exist during translocation into a nanopore because their large and coiled structure needs to unwind. In this work, we investigated DNA translocation dynamics through a 200 nm pore using a fast photon counting system (FPCS). We found that the dwell time of the DNA molecules depends on the inverse of voltage (tau proportional to V-1.02) with a large constant term (similar to 1 ms). In other words, spherical fluorescence bead translocation involves electrophoresis as well as other additional factors. Our theoretical calculation suggested that one additional factor is electro-osmotic trapping associated with the instantaneous Brownian motion before and after translocation. Furthermore, compressed DNA molecular conformation was seen as a result of the increase of peak photon counts and the decrease of electrophoretic mobility with voltage. Our experiments showed that the polymers at the vicinity of a nanopore can be trapped and compressed, which is necessary to understand how to control the polymer translocation into a nanopore.
Web of Science ® 被引用回数 : 3
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- DOI : 10.1039/c9an01253b
- ISSN : 0003-2654
- eISSN : 1364-5528
- Web of Science ID : WOS:000484974900006