論文

査読有り 本文へのリンクあり
2017年7月5日

Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution

Cell Reports
  • Shimpei I. Kubota
  • ,
  • Kei Takahashi
  • ,
  • Jun Nishida
  • ,
  • Yasuyuki Morishita
  • ,
  • Shogo Ehata
  • ,
  • Kazuki Tainaka
  • ,
  • Kohei Miyazono
  • ,
  • Hiroki R. Ueda

20
1
開始ページ
236
終了ページ
250
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.celrep.2017.06.010
出版者・発行元
CELL PRESS

Stochastic and proliferative events initiated from a single cell can disrupt homeostatic balance and lead to fatal disease processes such as cancer metastasis. To overcome metastasis, it is necessary to detect and quantify sparsely distributed metastatic cells throughout the body at early stages. Here, we demonstrate that clear, unobstructed brain/body imaging cocktails and computational analysis (CUBIC)-based cancer (CUBIC-cancer) analysis with a refractive index (RI)-optimized protocol enables comprehensive cancer cell profiling of the whole body and organs. We applied CUBIC-cancer analysis to 13 mouse models using nine cancer cell lines and spatiotemporal quantification of metastatic cancer progression at single-cell resolution. CUBIC-cancer analysis suggests that the epithelial-mesenchymal transition promotes not only extravasation but also cell survival at metastatic sites. CUBIC-cancer analysis is also applicable to pharmacotherapeutic profiling of anti-tumor drugs. CUBIC-cancer analysis is compatible with in vivo bioluminescence imaging and 2D histology. We suggest that a scalable analytical pipeline with these three modalities may contribute to addressing currently incurable metastatic diseases.

Web of Science ® 被引用回数 : 110

リンク情報
DOI
https://doi.org/10.1016/j.celrep.2017.06.010
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28683317
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000404899700021&DestApp=WOS_CPL
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85021811231&origin=inward 本文へのリンクあり
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85021811231&origin=inward
ID情報
  • DOI : 10.1016/j.celrep.2017.06.010
  • ISSN : 2211-1247
  • eISSN : 2211-1247
  • PubMed ID : 28683317
  • SCOPUS ID : 85021811231
  • Web of Science ID : WOS:000404899700021

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