論文

査読有り 本文へのリンクあり 国際誌
2018年8月21日

Chemical Landscape for Tissue Clearing Based on Hydrophilic Reagents

Cell Reports
  • Kazuki Tainaka
  • ,
  • Tatsuya C. Murakami
  • ,
  • Etsuo A. Susaki
  • ,
  • Chika Shimizu
  • ,
  • Rie Saito
  • ,
  • Kei Takahashi
  • ,
  • Akiko Hayashi-Takagi
  • ,
  • Hiroshi Sekiya
  • ,
  • Yasunobu Arima
  • ,
  • Satoshi Nojima
  • ,
  • Masako Ikemura
  • ,
  • Tetsuo Ushiku
  • ,
  • Yoshihiro Shimizu
  • ,
  • Masaaki Murakami
  • ,
  • Kenji F. Tanaka
  • ,
  • Masamitsu Iino
  • ,
  • Haruo Kasai
  • ,
  • Toshikuni Sasaoka
  • ,
  • Kazuto Kobayashi
  • ,
  • Kohei Miyazono
  • ,
  • Eiichi Morii
  • ,
  • Tadashi Isa
  • ,
  • Masashi Fukayama
  • ,
  • Akiyoshi Kakita
  • ,
  • Hiroki R. Ueda

24
8
開始ページ
2196
終了ページ
2210.e9
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.celrep.2018.07.056

We describe a strategy for developing hydrophilic chemical cocktails for tissue delipidation, decoloring, refractive index (RI) matching, and decalcification, based on comprehensive chemical profiling. More than 1,600 chemicals were screened by a high-throughput evaluation system for each chemical process. The chemical profiling revealed important chemical factors: salt-free amine with high octanol/water partition-coefficient (logP) for delipidation, N-alkylimidazole for decoloring, aromatic amide for RI matching, and protonation of phosphate ion for decalcification. The strategic integration of optimal chemical cocktails provided a series of CUBIC (clear, unobstructed brain/body imaging cocktails and computational analysis) protocols, which efficiently clear mouse organs, mouse body including bone, and even large primate and human tissues. The updated CUBIC protocols are scalable and reproducible, and they enable three-dimensional imaging of the mammalian body and large primate and human tissues. This strategy represents a future paradigm for the rational design of hydrophilic clearing cocktails that can be used for large tissues. Tainaka et al. describe a strategy for developing hydrophilic tissue-clearing reagents based on comprehensive chemical profiling. The strategic integration of optimal chemical cocktails provided a series of CUBIC protocols, which enable the 3D imaging of the mammalian body and large primate and human tissues.

リンク情報
DOI
https://doi.org/10.1016/j.celrep.2018.07.056
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30134179
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85051499044&origin=inward 本文へのリンクあり
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85051499044&origin=inward
ID情報
  • DOI : 10.1016/j.celrep.2018.07.056
  • eISSN : 2211-1247
  • PubMed ID : 30134179
  • SCOPUS ID : 85051499044

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