論文

査読有り 本文へのリンクあり 国際誌
2017年9月

ZEB1-regulated inflammatory phenotype in breast cancer cells

Molecular Oncology
  • Akihiro Katsura
  • ,
  • Yusuke Tamura
  • ,
  • Satoshi Hokari
  • ,
  • Mayumi Harada
  • ,
  • Masato Morikawa
  • ,
  • Tsubasa Sakurai
  • ,
  • Kei Takahashi
  • ,
  • Anna Mizutani
  • ,
  • Jun Nishida
  • ,
  • Yuichiro Yokoyama
  • ,
  • Yasuyuki Morishita
  • ,
  • Takashi Murakami
  • ,
  • Shogo Ehata
  • ,
  • Kohei Miyazono
  • ,
  • Daizo Koinuma

11
9
開始ページ
1241
終了ページ
1262
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/1878-0261.12098

Zinc finger E-box binding protein 1 (ZEB1) and ZEB2 induce epithelial-mesenchymal transition (EMT) and enhance cancer progression. However, the global view of transcriptional regulation by ZEB1 and ZEB2 is yet to be elucidated. Here, we identified a ZEB1-regulated inflammatory phenotype in breast cancer cells using chromatin immunoprecipitation sequencing and RNA sequencing, followed by gene set enrichment analysis (GSEA) of ZEB1-bound genes. Knockdown of ZEB1 and/or ZEB2 resulted in the downregulation of genes encoding inflammatory cytokines related to poor prognosis in patients with cancer, including IL6 and IL8, therefore suggesting that ZEB1 and ZEB2 have similar functions in terms of the regulation of production of inflammatory cytokines. Antibody array and ELISA experiments confirmed that ZEB1 controlled the production of the IL-6 and IL-8 proteins. The secretory proteins regulated by ZEB1 enhanced breast cancer cell proliferation and tumor growth. ZEB1 expression in breast cancer cells also affected the growth of fibroblasts in cell culture, and the accumulation of myeloid-derived suppressor cells in tumors in vivo. These findings provide insight into the role of ZEB1 in the progression of cancer, mediated by inflammatory cytokines, along with the initiation of EMT.

リンク情報
DOI
https://doi.org/10.1002/1878-0261.12098
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28618162
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5579340
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85022188359&origin=inward 本文へのリンクあり
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85022188359&origin=inward
ID情報
  • DOI : 10.1002/1878-0261.12098
  • ISSN : 1574-7891
  • eISSN : 1878-0261
  • PubMed ID : 28618162
  • PubMed Central 記事ID : PMC5579340
  • SCOPUS ID : 85022188359

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