2020年12月18日
Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing
STAR Protocols
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- 巻
- 1
- 号
- 3
- 開始ページ
- 100191
- 終了ページ
- 100191
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.xpro.2020.100191
Tissue-clearing technologies have developed rapidly in the past decade, especially for use in neuroscience research. We previously reported that CUBIC, which is one tissue-clearing method, is useful for applications in cancer research. CUBIC cancer analysis can be used to detect cancer metastasis with single-cell resolution at whole mouse body/organ level. This approach can also analyze the tumor characteristics with high-quality 3D images. Here, we describe a detailed CUBIC cancer protocol from tissue clearing, capturing 3D images and post-immunohistochemistry. For complete details on the use and execution of this protocol, please refer to Kubota et al. (2017).
- リンク情報
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- DOI
- https://doi.org/10.1016/j.xpro.2020.100191
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/33377085
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757565
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85108291151&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85108291151&origin=inward
- ID情報
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- DOI : 10.1016/j.xpro.2020.100191
- eISSN : 2666-1667
- PubMed ID : 33377085
- PubMed Central 記事ID : PMC7757565
- SCOPUS ID : 85108291151