論文

査読有り 本文へのリンクあり 国際誌
2020年12月18日

Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing

STAR Protocols
  • Kei Takahashi
  • ,
  • Shimpei I. Kubota
  • ,
  • Shogo Ehata
  • ,
  • Hiroki R. Ueda
  • ,
  • Kohei Miyazono

1
3
開始ページ
100191
終了ページ
100191
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.xpro.2020.100191

Tissue-clearing technologies have developed rapidly in the past decade, especially for use in neuroscience research. We previously reported that CUBIC, which is one tissue-clearing method, is useful for applications in cancer research. CUBIC cancer analysis can be used to detect cancer metastasis with single-cell resolution at whole mouse body/organ level. This approach can also analyze the tumor characteristics with high-quality 3D images. Here, we describe a detailed CUBIC cancer protocol from tissue clearing, capturing 3D images and post-immunohistochemistry. For complete details on the use and execution of this protocol, please refer to Kubota et al. (2017).

リンク情報
DOI
https://doi.org/10.1016/j.xpro.2020.100191
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33377085
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757565
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85108291151&origin=inward 本文へのリンクあり
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85108291151&origin=inward
ID情報
  • DOI : 10.1016/j.xpro.2020.100191
  • eISSN : 2666-1667
  • PubMed ID : 33377085
  • PubMed Central 記事ID : PMC7757565
  • SCOPUS ID : 85108291151

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