論文

査読有り 本文へのリンクあり 国際誌
2021年

Whole-organ analysis of TGF-β-mediated remodelling of the tumour microenvironment by tissue clearing

Communications Biology
  • Shimpei I. Kubota
  • ,
  • Kei Takahashi
  • ,
  • Tomoyuki Mano
  • ,
  • Katsuhiko Matsumoto
  • ,
  • Takahiro Katsumata
  • ,
  • Shoi Shi
  • ,
  • Kazuki Tainaka
  • ,
  • Hiroki R. Ueda
  • ,
  • Shogo Ehata
  • ,
  • Kohei Miyazono

4
1
開始ページ
294
終了ページ
294
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s42003-021-01786-y

Tissue clearing is one of the most powerful strategies for a comprehensive analysis of disease progression. Here, we established an integrated pipeline that combines tissue clearing, 3D imaging, and machine learning and applied to a mouse tumour model of experimental lung metastasis using human lung adenocarcinoma A549 cells. This pipeline provided the spatial information of the tumour microenvironment. We further explored the role of transforming growth factor-β (TGF-β) in cancer metastasis. TGF-β-stimulated cancer cells enhanced metastatic colonization of unstimulated-cancer cells in vivo when both cells were mixed. RNA-sequencing analysis showed that expression of the genes related to coagulation and inflammation were up-regulated in TGF-β-stimulated cancer cells. Further, whole-organ analysis revealed accumulation of platelets or macrophages with TGF-β-stimulated cancer cells, suggesting that TGF-β might promote remodelling of the tumour microenvironment, enhancing the colonization of cancer cells. Hence, our integrated pipeline for 3D profiling will help the understanding of the tumour microenvironment.

リンク情報
DOI
https://doi.org/10.1038/s42003-021-01786-y
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33674758
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935961
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85102203031&origin=inward 本文へのリンクあり
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85102203031&origin=inward
ID情報
  • DOI : 10.1038/s42003-021-01786-y
  • eISSN : 2399-3642
  • PubMed ID : 33674758
  • PubMed Central 記事ID : PMC7935961
  • SCOPUS ID : 85102203031

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