論文

査読有り 国際誌
2015年7月

Mammary tissue microenvironment determines T cell-dependent breast cancer-associated inflammation.

Cancer science
  • Kei Takahashi
  • ,
  • Nao Nagai
  • ,
  • Keisuke Ogura
  • ,
  • Koichi Tsuneyama
  • ,
  • Ikuo Saiki
  • ,
  • Tatsuro Irimura
  • ,
  • Yoshihiro Hayakawa

106
7
開始ページ
867
終了ページ
74
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/cas.12685

Although the importance of the host tissue microenvironment in cancer progression and metastasis has been established, the spatiotemporal process establishing a cancer metastasis-prone tissue microenvironment remains unknown. In this study, we aim to understand the immunological character of a metastasis-prone microenvironment in a murine 4T1 breast tumor model, by using the activation of nuclear factor-κb (NF-κB) in cancer cells as a sensor of inflammatory status and by monitoring its activity by bioluminescence imaging. By using a 4T1 breast cancer cell line stably expressing an NF-κB/Luc2 reporter gene (4T1 NF-κB cells), we observed significantly increased bioluminescence approximately 7 days after metastasis-prone orthotopic mammary fat-pad inoculation but not ectopic s.c. inoculation of 4T1 NF-κB cells. Such in vivo NF-κB activation within the fat-pad 4T1 tumor was diminished in immune-deficient SCID or nude mice, or T cell-depleted mice, suggesting the requirement of host T cell-mediated immune responses. Given the fat-pad 4T1 tumor expressed higher inflammatory mediators in a T cell-dependent mechanism compared to the s.c. tumor, our results imply the importance of the surrounding tissue microenvironment for inflaming tumors by collaborating with T cells to instigate metastatic spread of 4T1 breast cancer cells.

リンク情報
DOI
https://doi.org/10.1111/cas.12685
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25940224
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4520638
ID情報
  • DOI : 10.1111/cas.12685
  • PubMed ID : 25940224
  • PubMed Central 記事ID : PMC4520638

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