2017年12月
Phytoestrogens Weaken the Blood-Milk Barrier in Lactating Mammary Epithelial Cells by Affecting Tight Junctions and Cell Viability
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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- 巻
- 65
- 号
- 50
- 開始ページ
- 11118
- 終了ページ
- 11124
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/acs.jafc.7b04786
- 出版者・発行元
- AMER CHEMICAL SOC
During lactation, mammary epithelial cells (MECs) form the blood-milk barrier by less-permeable tight junctions (TJs) to prevent the leakage of milk components. Phytoestrogens affect the proliferation, differentiation, and apoptosis of MECs. However, it remains unclear whether phytoestrogens are involved in the blood-milk barrier. Therefore, we investigated the influence of phytoestrogens (coumestrol, genistein, and daidzein) by using an in vitro mouse-MEC-culture model. The results showed that coumestrol and genistein changed the expression of TJ proteins (claudins-3 and -4 and occludin), weakened barrier function, and reduced beta-casein production. Daidzein also weakened barrier function without inhibiting beta-casein production. Additionally, coumestrol and genistein induced apoptosis in MECs. These results indicate that phytoestrogens weaken the blood-milk barrier by directly affecting TJs and the cellular viability of lactating MECs in different ways.
- リンク情報
- ID情報
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- DOI : 10.1021/acs.jafc.7b04786
- ISSN : 0021-8561
- eISSN : 1520-5118
- Web of Science ID : WOS:000418783400032