2013年5月
Rab12 regulates mTORC1 activity and autophagy through controlling the degradation of amino-acid transporter PAT4
EMBO Reports
- ,
- 巻
- 14
- 号
- 5
- 開始ページ
- 450
- 終了ページ
- 457
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/embor.2013.32
Autophagy is an evolutionarily conserved catabolic mechanism that targets intracellular molecules and damaged organelles to lysosomes. Autophagy is achieved by a series of membrane trafficking events, but their regulatory mechanisms are poorly understood. Here, we report small GTPase Rab12 as a new type of autophagic regulator that controls the degradation of an amino-acid transporter. Knockdown of Rab12 results in inhibition of autophagy and in increased activity of mTORC1 (mammalian/mechanistic target of rapamycin complex 1), an upstream regulator of autophagy. We also found that Rab12 promotes constitutive degradation of PAT4 (proton-coupled amino-acid transporter 4), whose accumulation in Rab12-knockdown cells modulates mTORC1 activity and autophagy. Our findings reveal a new mechanism of regulation of mTORC1 signalling and autophagy, that is, quality control of PAT4 by Rab12. © 2013 European Molecular Biology Organizatio.
- ID情報
-
- DOI : 10.1038/embor.2013.32
- ISSN : 1469-221X
- ISSN : 1469-3178
- PubMed ID : 23478338
- SCOPUS ID : 84877582117