2013年4月
TRAP Display: A High-Speed Selection Method for the Generation of Functional Polypeptides
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
- ,
- ,
- ,
- 巻
- 135
- 号
- 14
- 開始ページ
- 5433
- 終了ページ
- 5440
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/ja312579u
- 出版者・発行元
- AMER CHEMICAL SOC
Here, we describe a novel method that enables highspeed in vitro selection of functional peptides, peptidomimetics, and proteins via a simple procedure. We first developed a new cell-free translation system, the TRAP system (transcription translation coupled with association of Euromycin linker), which automatically produces a polypeptide library through a series of sequential reactions: transcription, association of puromycin-DNA linker, translation, and conjugation between the nascent polypeptide and puromycin-DNA linker. We then applied the TRAP system for the selection of macrocyclic peptides against human serum albumin. Six rounds of selection using TRAP display were performed in approximately 14 h, yielding macrocyclic peptides with nanomolar affinity to their target protein. Because TRAP display enables high-speed selection of functional polypeptides, it will facilitate the generation of various polypeptides that are useful for biological and therapeutic applications.
- リンク情報
- ID情報
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- DOI : 10.1021/ja312579u
- ISSN : 0002-7863
- PubMed ID : 23506421
- Web of Science ID : WOS:000317548400045