論文

査読有り
2010年8月

Ectopic Reelin Induces Neuronal Aggregation with a Normal Birthdate-Dependent "Inside-Out" Alignment in the Developing Neocortex

JOURNAL OF NEUROSCIENCE
  • Ken-ichiro Kubo
  • ,
  • Takao Honda
  • ,
  • Kenji Tomita
  • ,
  • Katsutoshi Sekine
  • ,
  • Kazuhiro Ishii
  • ,
  • Asuka Uto
  • ,
  • Kazuma Kobayashi
  • ,
  • Hidenori Tabata
  • ,
  • Kazunori Nakajima

30
33
開始ページ
10953
終了ページ
10966
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1523/JNEUROSCI.0486-10.2010
出版者・発行元
SOC NEUROSCIENCE

Neurons in the developing mammalian neocortex form the cortical plate (CP) in an "inside-out" manner; that is, earlier-born neurons form the deeper layers, whereas later-born neurons migrate past the existing layers and form the more superficial layers. Reelin, a glycoprotein secreted by Cajal-Retzius neurons in the marginal zone (MZ), is crucial for this "inside-out" layering, because the layers are inverted in the Reelin-deficient mouse, reeler (Relnrl). Even though more than a decade has passed since the discovery of reelin, the biological effect of Reelin on individual migrating neurons remains unclear. In addition, although the MZ is missing in the reeler cortex, it is unknown whether Reelin directly regulates the development of the cell-body-sparse MZ. To address these issues, we expressed Reelin ectopically in the developing mouse cortex, and the results showed that Reelin caused the leading processes of migrating neurons to assemble in the Reelin-rich region, which in turn induced their cell bodies to form cellular aggregates around Reelin. Interestingly, the ectopic Reelin-rich region became cell-body-sparse and dendrite-rich, resembling the MZ, and the late-born neurons migrated past their predecessors toward the central Reelin-rich region within the aggregates, resulting in a birthdate-dependent "inside-out" alignment even ectopically. Reelin receptors and intracellular adaptor protein Dab1 were found to be necessary for formation of the aggregates. The above findings indicate that Reelin signaling is capable of inducing the formation of the dendrite-rich, cell-body-sparse MZ and a birthdate-dependent "inside-out" alignment of neurons independently of other factors/structures near the MZ.

リンク情報
DOI
https://doi.org/10.1523/JNEUROSCI.0486-10.2010
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000281197900001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1523/JNEUROSCI.0486-10.2010
  • ISSN : 0270-6474
  • Web of Science ID : WOS:000281197900001

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