論文

査読有り 責任著者 本文へのリンクあり 国際誌
2024年6月

Focal brain cooling suppresses spreading depolarization and reduces endothelial nitric oxide synthase expression in rats.

IBRO neuroscience reports
  • Yuya Hirayama
  • ,
  • Hiroyuki Kida
  • ,
  • Takao Inoue
  • ,
  • Kazutaka Sugimoto
  • ,
  • Fumiaki Oka
  • ,
  • Satoshi Shirao
  • ,
  • Hirochika Imoto
  • ,
  • Sadahiro Nomura
  • ,
  • Michiyasu Suzuki

16
開始ページ
609
終了ページ
621
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.ibneur.2024.05.001

This study aimed to investigate the effects of focal brain cooling (FBC) on spreading depolarization (SD), which is associated with several neurological disorders. Although it has been studied from various aspects, no medication has been developed that can effectively control SD. As FBC can reduce neuronal damage and promote functional recovery in pathological conditions such as epilepsy, cerebral ischemia, and traumatic brain injury, it may also potentially suppress the onset and progression of SD. We created an experimental rat model of SD by administering 1 M potassium chloride (KCl) to the cortical surface. Changes in neuronal and vascular modalities were evaluated using multimodal recording, which simultaneously recorded brain temperature (BrT), wide range electrocorticogram, and two-dimensional cerebral blood flow. The rats were divided into two groups (cooling [CL] and non-cooling [NC]). Warm or cold saline was perfused on the surface of one hemisphere to maintain BrT at 37°C or 15°C in the NC and CL groups, respectively. Western blot analysis was performed to determine the effects of FBC on endothelial nitric oxide synthase (eNOS) expression. In the NC group, KCl administration triggered repetitive SDs (mean frequency = 11.57/h). In the CL group, FBC increased the duration of all KCl-induced events and gradually reduced their frequency. Additionally, eNOS expression decreased in the cooled brain regions compared to the non-cooled contralateral hemisphere. The results obtained by multimodal recording suggest that FBC suppresses SD and decreases eNOS expression. This study may contribute to developing new treatments for SD and related neurological disorders.

リンク情報
DOI
https://doi.org/10.1016/j.ibneur.2024.05.001 本文へのリンクあり
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/38800086
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11127172
ID情報
  • DOI : 10.1016/j.ibneur.2024.05.001
  • PubMed ID : 38800086
  • PubMed Central 記事ID : PMC11127172

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