2021年12月11日
Chemical and Microbial Characteristics of Blackening Disease in Lotus (Nelumbo nucifera Gaertn.) Caused by Hirschmanniella diversa Sher
Agronomy
- 巻
- 11
- 号
- 12
- 開始ページ
- 2517
- 終了ページ
- 2517
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3390/agronomy11122517
- 出版者・発行元
- MDPI AG
The lotus (Nelumbo nucifera Gaertn.) is widely cultivated in Asia, but a blackening disease in the lotus tuber, called “kurokawa-senchu-byo”, is a serious problem caused by the Hirschmanniella diversa Sher plant-parasitic nematode. To effectively control the disease, we must elucidate the blackening mechanisms; therefore, in this study, we performed a soil chemical analysis and an evaluation of the disease level in the lotus cultivation fields, identified the chemical components of the black spots on the lotus surface, and performed a 16S rRNA gene-based microbial community analysis of the black spots. Using linear regression analysis, a positive linear relationship with a strong correlation between the damage index values and fertilizer components such as P2O5 was observed. As a result of scanning electron microscopy and energy-dispersive X-ray spectroscopy analysis, phosphorus (P) and iron (Fe) were found to be concentrated in the black spots of the lotus tubers. Furthermore, we found that the concentrations of P and Fe in the black spots were 1.5- and 2.7-fold higher, respectively, than those found in the healthy parts of the lotus tubers. A 16S rRNA gene analysis revealed that dissimilatory Fe(III)-reducing bacteria (DIRB) were predominant in the black spots, suggesting that these bacteria are important to the formation of P and Fe compounds in the black spots.
- ID情報
-
- DOI : 10.3390/agronomy11122517
- eISSN : 2073-4395