2012年2月14日
Conserved role for the Dachshund protein with Drosophila Pax6 homolog eyeless in insulin expression
Proceedings of the National Academy of Sciences of the United States of America
- ,
- ,
- 巻
- 109
- 号
- 7
- 開始ページ
- 2406
- 終了ページ
- 2411
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1073/pnas.1116050109
- 出版者・発行元
- Proceedings of the National Academy of Sciences
Members of the insulin family peptides have conserved roles in the regulation of growth and metabolism in a wide variety of metazoans. The Drosophila genome encodes seven insulin-like peptide genes, dilp1-7, and the most prominent dilps (dilp2, dilp3, and dilp5) are expressed in brain neurosecretory cells known as "insulin-producing cells" (IPCs). Although these dilps are expressed in the same cells, the expression of each dilp is regulated independently. However, the molecular mechanisms that regulate the expression of individual dilps in the IPCs remain largely unknown. Here, we show that Dachshund (Dac), which is a highly conserved nuclear protein, is a critical transcription factor that specifically regulates dilp5 expression. Dac was strongly expressed in IPCs throughout development. dac loss-of-function analyses revealed a severely reduced dilp5 expression level in young larvae. Dac interacted physically with the Drosophila Pax6 homolog Eyeless (Ey), and these proteins synergistically promoted dilp5 expression. In addition, the mammalian homolog of Dac, Dach1/2, facilitated the promoting action of Pax6 on the expression of islet hormone genes in cultured mammalian cells. These observations indicate the conserved role of Dac/Dach in controlling insulin expression in conjunction with Ey/Pax6.
- リンク情報
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- DOI
- https://doi.org/10.1073/pnas.1116050109
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/22308399
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000300489200052&DestApp=WOS_CPL
- URL
- https://syndication.highwire.org/content/doi/10.1073/pnas.1116050109
- ID情報
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- DOI : 10.1073/pnas.1116050109
- ISSN : 0027-8424
- ISSN : 1091-6490
- eISSN : 1091-6490
- ORCIDのPut Code : 36284865
- PubMed ID : 22308399
- SCOPUS ID : 84857127737
- Web of Science ID : WOS:000300489200052