論文

2022年2月16日

Examination of Cyp51A-Mediated Azole Resistance in Aspergillus lentulus Using CRISPR/Cas9 Genome Editing.

Medical mycology journal
  • Madoka Tateno
  • ,
  • Takashi Umeyama
  • ,
  • Tatsuya Inukai
  • ,
  • Shogo Takatsuka
  • ,
  • Yasutaka Hoshino
  • ,
  • Satoshi Yamagoe
  • ,
  • Somay Yamagata Murayama
  • ,
  • Keiko Ishino
  • ,
  • Yoshitsugu Miyazaki

63
2
開始ページ
27
終了ページ
35
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3314/mmj.21-00024

Aspergillus lentulus was first reported in 2005 as a cryptic species of Aspergillus fumigatus, and since then, its resistance to azole drugs and the high mortality rate of infected individuals have emerged as problems. Although it has been reported that P450 14-α sterol demethylase (Cyp51) is involved in azole resistance in A. lentulus, the specific resistance mechanism has not been elucidated. In this study, we successfully introduced the entire A. fumigatus cyp51A gene into the cyp51A locus in A. lentulus using the CRISPR/Cas9 genome-editing system. The A. lentulus strains harboring A. fumigatus cyp51A showed reduced minimum inhibitory concentrations for itraconazole and voriconazole compared with those of the parent strain. This finding suggests that Cyp51A is involved in azole resistance in A. lentulus and may contribute to the elucidation of the mechanism of resistance to azole drugs via Cyp51A and to the development of new antifungal drugs. In addition, our successful application of the CRISPR/Cas9 system to A. lentulus opens the door to examination of other gene functions in this fungus.

リンク情報
DOI
https://doi.org/10.3314/mmj.21-00024
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/35173102
ID情報
  • DOI : 10.3314/mmj.21-00024
  • PubMed ID : 35173102

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