論文

国際誌
2021年

Optogenetic Potentials of Diverse Animal Opsins: Parapinopsin, Peropsin, LWS Bistable Opsin.

Advances in experimental medicine and biology
  • Mitsumasa Koyanagi
  • ,
  • Tomoka Saito
  • ,
  • Seiji Wada
  • ,
  • Takashi Nagata
  • ,
  • Emi Kawano-Yamashita
  • ,
  • Akihisa Terakita

1293
開始ページ
141
終了ページ
151
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/978-981-15-8763-4_8

Animal opsin-based pigments are light-activated G-protein-coupled receptors (GPCRs), which drive signal transduction cascades via G-proteins. Thousands of animal opsins have been identified, and molecular phylogenetic and biochemical analyses have revealed the unexpected diversity in selectivity of G-protein activation and photochemical property. Here we discuss the optogenetic potentials of diverse animal opsins, particularly recently well-characterized three non-canonical opsins, parapinopsin, peropsin, and LWS bistable opsin. Unlike canonical opsins such as vertebrate visual opsins that have been conventionally used for optogenetic applications, these opsins are bistable; opsin-based pigments do not release the chromophore retinal after light absorption, and the stable photoproducts revert to their original dark states upon subsequent light absorption. Parapinopsins have a "complete photoregeneration ability," which allows a clear color-dependent regulation of signal transductions. On the other hand, peropsins serve as a "dark-active and light-inactivated" GPCR to regulate signal transductions in the opposite way compared with usual opsins. In addition, an LWS bistable opsin from a butterfly was revealed to be the longest wavelength-sensitive animal opsin with its absorption maximum at ~570 nm. The property-dependent optical regulations of signal transductions were demonstrated in mammalian cultured cells, showing potentials of new optogenetic tools.

リンク情報
DOI
https://doi.org/10.1007/978-981-15-8763-4_8
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33398811
ID情報
  • DOI : 10.1007/978-981-15-8763-4_8
  • ORCIDのPut Code : 82579596
  • PubMed ID : 33398811

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