2009年5月
Determination of 1-nitropyrene in low volume ambient air samples by high-performance liquid chromatography with fluorescence detection
JOURNAL OF CHROMATOGRAPHY A
- ,
- ,
- ,
- ,
- 巻
- 1216
- 号
- 21
- 開始ページ
- 4625
- 終了ページ
- 4628
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.chroma.2009.03.070
- 出版者・発行元
- ELSEVIER SCIENCE BV
To measure the actual exposure of a person to 1-nitropyrene (1-NP) in airborne particulate matter, it is considered more accurate to collect air samples with a portable air sampler than to sample at a fixed location. However, because the portable samplers can sample only small volumes, a sensitive method is needed to analyze the Compounds that are collected on a filter. Here we describe a high-performance liquid chromatographic (HPLC) method with fluorescence detection that is sensitive and precise enough for use with portable air samplers. The developed column-switching system Successfully removed the interfering Substances in the samples with only a simple pretreatment. To improve the precision of the measurement. cleuterated 1-NP was used as an internal standard, and it eluted immediately prior to 1-NP with sufficient resolution (R(s) 1.50). The detection limit was 0.32 fmol/injection, and the calibration range was from 2 to 100 fmol. The proposed method was applied to determining 1-NP in fine airborne particulate matter (PM(2.5)) at two sites with low pollution levels. 1-NP was detected in all samples at concentrations in the low fmol/m(3) range. The proposed method has enough sensitivity and precision to determine 1-NP in the limited air volume of the portable sampler. (C) 2009 Elsevier B.V. All rights reserved.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.chroma.2009.03.070
- ISSN : 0021-9673
- CiNii Articles ID : 120001342275
- Web of Science ID : WOS:000266175100013