2009年9月
Determination of airborne particle-associated benz[a]anthracene-7,12-quinone using high-performance liquid chromatography with in-line reduction and fluorescence detection
JOURNAL OF CHROMATOGRAPHY A
- ,
- ,
- ,
- ,
- 巻
- 1216
- 号
- 39
- 開始ページ
- 6758
- 終了ページ
- 6761
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.chroma.2009.08.009
- 出版者・発行元
- ELSEVIER SCIENCE BV
A simple and sensitive method for measuring airborne particle-associated benz[a]anthracene-7,12-quinone (BaAQ) based on two-dimensional high-performance liquid chromatography (HPLC) with fluorescence detection was established. The system involves an ODS column for sample cleanup, a 6-port switching valve. an ODS column for trapping the fraction containing BaAQ, an ODS column for sample separation, and a Pt-Rh catalytic column for reduction of BaAQ to a corresponding fluorescent compound. The accuracy of the assay, as applied to airborne particulate sample extracts spiked with known amounts of BaAQ was 94-108%. The detection limit was 97 fmol per injection (signal-to-noise ratio = 3), and the calibration range was from I to 100 pmol with excellent proportionality (R(2) = 0.9995). BaAQ in airborne particles collected in Osaka, Japan every 3 h was successfully measured using the established analytical method with simple sample preparation steps: ultrasonic extraction in organic solvent and concentration under reduced pressure and/or nitrogen stream. (C) 2009 Elsevier B.V. All rights reserved.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.chroma.2009.08.009
- ISSN : 0021-9673
- Web of Science ID : WOS:000269846100012