2009年6月
Multi-focus excitation coherent anti-Stokes Raman scattering (CARS) microscopy and its applications for real-time imaging
OPTICS EXPRESS
- ,
- ,
- ,
- ,
- 巻
- 17
- 号
- 12
- 開始ページ
- 9526
- 終了ページ
- 9536
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1364/OE.17.009526
- 出版者・発行元
- OPTICAL SOC AMER
We developed a multi-focus excitation coherent anti-Stokes Raman scattering (CARS) microscope using a microlens array scanner for real-time molecular imaging. Parallel exposure of a specimen with light from two highly controlled picosecond mode-locked lasers (jitter of 30 fs through an electronic low-pass filter with 150 Hz bandwidth, point-by-point wavelength scan within 300 ms) and parallel detection with an image sensor enabled real-time imaging. We demonstrated real-time CARS imaging of polystyrene beads (frame rate of 30 fps), a giant multi-lamellar vesicle of dipalmitoylphosphatidylcholine (frame rate of 10 fps), and living HeLa cells (frame rate of 10 fps). (C) 2009 Optical Society of America
- リンク情報
- ID情報
-
- DOI : 10.1364/OE.17.009526
- ISSN : 1094-4087
- PubMed ID : 19506600
- Web of Science ID : WOS:000266906400006