2016年3月
Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing Agent
CELL REPORTS
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 14
- 号
- 11
- 開始ページ
- 2718
- 終了ページ
- 2732
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.celrep.2016.02.057
- 出版者・発行元
- CELL PRESS
Super-resolution imaging deep inside tissues has been challenging, as it is extremely sensitive to light scattering and spherical aberrations. Here, we report an optimized optical clearing agent for high-resolution fluorescence imaging (SeeDB2). SeeDB2 matches the refractive indices of fixed tissues to that of immersion oil (1.518), thus minimizing both light scattering and spherical aberrations. During the clearing process, fine morphology and fluorescent proteins were highly preserved. SeeDB2 enabled super-resolution microscopy of various tissue samples up to a depth of > 100 mu m, an order of magnitude deeper than previously possible under standard mounting conditions. Using this approach, we demonstrate accumulation of inhibitory synapses on spine heads in NMDA-receptor-deficient neurons. In the fly medulla, we found unexpected heterogeneity in axon bouton orientations among Mi1 neurons, a part of the motion detection circuitry. Thus, volumetric super-resolution microscopy of cleared tissues is a powerful strategy in connectomic studies at synaptic levels.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.celrep.2016.02.057
- ISSN : 2211-1247
- PubMed ID : 26972009
- Web of Science ID : WOS:000372499000020