論文

国際誌
2020年10月22日

Higher genome mutation rates of Beijing lineage of Mycobacterium tuberculosis during human infection.

Scientific reports
  • Mariko Hakamata
  • ,
  • Hayato Takihara
  • ,
  • Tomotada Iwamoto
  • ,
  • Aki Tamaru
  • ,
  • Atsushi Hashimoto
  • ,
  • Takahiro Tanaka
  • ,
  • Shaban A Kaboso
  • ,
  • Gebremichal Gebretsadik
  • ,
  • Aleksandr Ilinov
  • ,
  • Akira Yokoyama
  • ,
  • Yuriko Ozeki
  • ,
  • Akihito Nishiyama
  • ,
  • Yoshitaka Tateishi
  • ,
  • Hiroshi Moro
  • ,
  • Toshiaki Kikuchi
  • ,
  • Shujiro Okuda
  • ,
  • Sohkichi Matsumoto

10
1
開始ページ
17997
終了ページ
17997
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-020-75028-2

Mycobacterium tuberculosis (Mtb) strains of Beijing lineage have caused great concern because of their rapid emergence of drug resistance and worldwide spread. DNA mutation rates that reflect evolutional adaptation to host responses and the appearance of drug resistance have not been elucidated in human-infected Beijing strains. We tracked and obtained an original Mtb isolate of Beijing lineage from the 1999 tuberculosis outbreak in Japan, as well as five other isolates that spread in humans, and two isolates from the patient caused recurrence. Three isolates were from patients who developed TB within one year after infection (rapid-progressor, RP), and the other three isolates were from those who developed TB more than one year after infection (slow-progressor, SP). We sequenced genomes of these isolates and analyzed the propensity and rate of genomic mutations. Generation time versus mutation rate curves were significantly higher for RP. The ratio of oxidative versus non-oxidation damages induced mutations was higher in SP than RP, suggesting that persistent Mtb are exposed to oxidative stress in the latent state. Our data thus demonstrates that higher mutation rates of Mtb Beijing strains during human infection is likely to account for the higher adaptability and an emergence ratio of drug resistance.

リンク情報
DOI
https://doi.org/10.1038/s41598-020-75028-2
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33093577
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582865
ID情報
  • DOI : 10.1038/s41598-020-75028-2
  • PubMed ID : 33093577
  • PubMed Central 記事ID : PMC7582865

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