論文

査読有り 国際誌
2018年10月5日

Development of an online two-dimensional high-performance liquid chromatographic system in combination with tandem mass spectrometric detection for enantiomeric analysis of free amino acids in human physiological fluid.

Journal of chromatography. A
  • Chiharu Ishii
  • ,
  • Takeyuki Akita
  • ,
  • Masashi Mita
  • ,
  • Tomomi Ide
  • ,
  • Kenji Hamase

1570
開始ページ
91
終了ページ
98
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.chroma.2018.07.076

An automated two-dimensional HPLC-MS/MS system was designed and developed for the highly selective determination of trace levels of d-amino acids. As the targets, frequently observed ones in mammalian physiological fluids, Ala, Asp, Glu, Leu, Pro and Ser, were selected because these d-amino acids are the potential biomarkers for the early and sensitive diagnoses of various diseases. The target analytes were derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), then isolated by a reversed-phase column in the first dimension. The NBD-amino acid fractions were automatically collected into the multi-loop device, and introduced into the enantioselective column in the second dimension to separate the d- and l-forms followed by detection by an MS/MS. The obtained resolution values of the enantiomers were 1.87-5.17 and the calibration lines, precision and accuracy were practically sufficient. By using the present 2D HPLC-MS/MS system, trace levels of d-amino acids in complex biological matrices were determined without disturbance by the intrinsic interfering compounds, and successfully applied to the analysis of the human clinical samples (plasma and urine).

Web of Science ® 被引用回数 : 29

リンク情報
DOI
https://doi.org/10.1016/j.chroma.2018.07.076
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30104059
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000445982400011&DestApp=WOS_CPL

エクスポート
BibTeX RIS