論文

国際誌
2019年9月17日

Three-Dimensional High-Performance Liquid Chromatographic Determination of Asn, Ser, Ala, and Pro Enantiomers in the Plasma of Patients with Chronic Kidney Disease.

Analytical chemistry
  • Aogu Furusho
  • ,
  • Reiko Koga
  • ,
  • Takeyuki Akita
  • ,
  • Masashi Mita
  • ,
  • Tomonori Kimura
  • ,
  • Kenji Hamase

91
18
開始ページ
11569
終了ページ
11575
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/acs.analchem.9b01615

The concentrations of several d-amino acids have been reported to significantly increase in the plasma of patients with chronic kidney disease (CKD). However, the amounts of these d-amino acids are low (around 1% of the l-form or lower), and their analyses were complicated by various interfering compounds in many clinical samples. A highly selective analytical method is thus required to perform the accurate and precise determination of these d-amino acids in the plasma of CKD patients. In the present study, a selective 3D-HPLC system equipped with reversed-phase, anion-exchange, and enantioselective columns has been designed and developed for the determination of the asparagine, serine, alanine and proline enantiomers. For the sensitive analysis, amino acids were precolumn derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole and detected by their fluorescence. The system was validated by using human plasma in addition to the standard amino acids, and results with a sufficient linearity, precision, and accuracy were obtained. The 3D-HPLC system was applied to the plasma of patients with different stages of CKD and all of the target d-amino acids were clearly observed without interferences for all 25 tested patients. Good correlations were shown between the kidney function of the patients and the %d values of the target analytes, especially d-Asn and d-Ser, indicating that the present 3D-HPLC method is useful for the sensitive diagnosis of CKD.

リンク情報
DOI
https://doi.org/10.1021/acs.analchem.9b01615
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31436409

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