論文

査読有り 筆頭著者 責任著者
2012年

Cultivable anaerobic microbiota of infected root canals

International Journal of Dentistry
  • Takuichi Sato
  • ,
  • Keiko Yamaki
  • ,
  • Naoko Ishida
  • ,
  • Kazuhiro Hashimoto
  • ,
  • Yasuhisa Takeuchi
  • ,
  • Megumi Shoji
  • ,
  • Emika Sato
  • ,
  • Junko Matsuyama
  • ,
  • Hidetoshi Shimauchi
  • ,
  • Nobuhiro Takahashi

2012
開始ページ
609689
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1155/2012/609689

Objective. Periapical periodontitis is an infectious and inflammatory disease of the periapical tissues caused by oral bacteria invading the root canal. In the present study, profiling of the microbiota in infected root canals was performed using anaerobic culture and molecular biological techniques for bacterial identification. Methods. Informed consent was obtained from all subjects (age ranges, 34-71 years). Nine infected root canals with periapical lesions from 7 subjects were included. Samples from infected root canals were collected, followed by anaerobic culture on CDC blood agar plates. After 7 days, colony forming units (CFU) were counted and isolated bacteria were identified by 16S rRNA gene sequencing. Results. The mean bacterial count (CFU) in root canals was (0.5 ± 1.1) 10 6 (range 8.0 × 10 1 - 3.1 × 10 6), and anaerobic bacteria were predominant (89.8%). The predominant isolates were Olsenella (25.4%), Mogibacterium (17.7%), Pseudoramibacter (17.7%), Propionibacterium (11.9%) and Parvimonas (5.9%). Conclusion. The combination of anaerobic culture and molecular biological techniques makes it possible to analyze rapidly the microbiota in infected root canals. The overwhelming majority of the isolates from infected root canals were found to be anaerobic bacteria, suggesting that the environment in root canals is anaerobic and therefore support the growth of anaerobes. © Copyright 2012 Takuichi Sato et al.

リンク情報
DOI
https://doi.org/10.1155/2012/609689
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22548063
ID情報
  • DOI : 10.1155/2012/609689
  • ISSN : 1687-8728
  • ISSN : 1687-8736
  • PubMed ID : 22548063
  • SCOPUS ID : 84861065084

エクスポート
BibTeX RIS